期刊论文详细信息
BMC Clinical Pathology
alphaB-crystallin is a marker of aggressive breast cancer behavior but does not independently predict for patient outcome: a combined analysis of two randomized studies
George Fountzilas6  Dimitrios Pectasides4  Ioannis Efstratiou1,10  George Pentheroudakis3  Dimosthenis V Skarlos1  Angelos Koutras1,14  Helen Gogas1,11  Anna Batistatou5  Christos Papadimitriou7  Irene Konstantopoulou2  Anastasia G Eleftheraki8  Vassiliki Kotoula9  Ioannis Kostopoulos1,13  Mattheos Bobos9  Flora Stavridi1,12  Triantafyllia Koletsa1,13 
[1] Second Department of Medical Oncology, “Metropolitan” Hospital, Piraeus, Greece;Molecular Diagnostics Laboratory, IRRP, National Centre for Scientific Research NCSR Demokritos, Athens, Greece;Department of Medical Oncology, Ioannina University Hospital, Ioannina, Greece;Second Department of Internal Medicine, Oncology Section, “Hippokration” Hospital, Athens, Greece;Department of Pathology, Ioannina University Hospital, Ioannina, Greece;Department of Medical Oncology, “Papageorgiou” Hospital, Aristotle University of Thessaloniki School of Medicine, Thessaloniki, Greece;Department of Clinical Therapeutics, “Alexandra” Hospital, University of Athens School of Medicine, Athens, Greece;Section of Biostatistics, Hellenic Cooperative Oncology Group, Data Office, Athens, Greece;Laboratory of Molecular Oncology, Hellenic Foundation for Cancer Research, Aristotle University of Thessaloniki School of Medicine, Thessaloniki, Greece;Department of Pathology, “Papageorgiou” Hospital, Thessaloniki, Greece;First Department of Medicine, “Laiko” General Hospital, University of Athens, Medical School, Athens, Greece;Third Department of Medical Oncology, “Hygeia” Hospital, Athens, Greece;Department of Pathology, Aristotle University of Thessaloniki School of Medicine, University Campus, 54124 Thessaloniki, Greece;Department of Medicine, Division of Oncology, University Hospital, University of Patras Medical School, Patras, Greece
关键词: Taxane-based therapy;    BRCA gene mutations;    Basal core phenotype;    Triple-negative breast cancer;    AlphaB-crystallin;    Breast cancer;   
Others  :  820441
DOI  :  10.1186/1472-6890-14-28
 received in 2013-07-10, accepted in 2014-06-12,  发布年份 2014
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【 摘 要 】

Background

alphaB-crystallin is a small heat shock protein that has recently been characterized as an oncoprotein correlating with the basal core phenotype and with negative prognostic factors in breast carcinomas. The purpose of this study was to evaluate alphaB-crystallin with respect to clinicopathological parameters and the outcome of patients with operable high-risk breast cancer.

Methods

A total of 940 tumors were examined, derived from an equal number of patients who had participated in two randomized clinical trials (paclitaxel-containing regimen in 793 cases). Immunohistochemistry for ER, PgR, HER2, Ki67, CK5, CK14, CK17, EGFR, alphaB-crystallin, BRCA1 and p53 was performed. BRCA1 mutation data were available in 89 cases.

Results

alphaβ-crystallin was expressed in 170 cases (18.1%) and more frequently in triple-negative breast carcinomas (TNBC) (45% vs. 14.5% non-TNBC, p < 0.001). alphaB-crystallin protein expression was significantly associated with high Ki67 (Pearson chi-square test, p < 0.001), p53 (p = 0.002) and basal cytokeratin protein expression (p < 0.001), BRCA1 mutations (p = 0.045) and negative ER (p < 0.001) and PgR (p < 0.001). Its overexpression, defined as >30% positive neoplastic cells, was associated with adverse overall survival (Wald’s p = 0.046). However, alphaB-crystallin was not an independent prognostic factor upon multivariate analysis. No interaction between taxane-based therapy and aβ-crystallin expression was observed.

Conclusions

In operable high-risk breast cancer, alphaB-crystallin protein expression is associated with poor prognostic features indicating aggressive tumor behavior, but it does not seem to have an independent impact on patient survival or to interfere with taxane-based therapy.

Trial registrations

ACTRN12611000506998 (HE10/97 trial) andACTRN12609001036202 (HE10/00 trial).

【 授权许可】

   
2014 Koletsa et al.; licensee BioMed Central Ltd.

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