Detection of hantavirus in bats from remaining rain forest in São Paulo, Brazil

BMC Research Notes

Edison Luiz Durigon²  Sandra Favorito¹  Caroline Cotrin Aires³  Lilia Mara Dutra²  Tatiana Ometto²  Daniele Lautenschalager²  Dyana Alves Henriques²  Luciano Matsumiya Thomazelli²  Jansen de Araujo² 

[1]Universidade Bandeirante de Sao Paulo - UNIBAN, Sao Paulo, Brazil

[2]BSL3+ Laboratory, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP CEP: 05508-900, Brazil

[3]Museu de Zoologia, Universidade de Sao Paulo, Sao Paulo, Brazil

关键词: Brazil;    Remaining rain forest;    Bats;    Wild rodents;    Hantavirus;   

Others  :  1165021
DOI  :  10.1186/1756-0500-5-690

 received in 2012-06-19, accepted in 2012-12-18,  发布年份 2012

【 摘 要 】

Background

The significant biodiversity found in Brazil is a potential for the emergence of new zoonoses. Study in some places of the world suggest of the presence to hantavirus in tissues of bats. Researches of hantavirus in wildlife, out rodents, are very scarce in Brazil. Therefore we decided to investigate in tissues of different species of wild animals captured in the same region where rodents were detected positive for this virus. The present work analyzed ninety-one animals (64 rodents, 19 opossums, and 8 bats) from a region of the Atlantic forest in Biritiba Mirin City, São Paulo State, Brazil. Lungs and kidneys were used for RNA extraction.

Findings

The samples were screened for evidence of hantavirus infection by SYBR-Green-based real-time RT-PCR. Sixteen samples positive were encountered among the wild rodents, bats, and opossums. The detection of hantavirus in the lungs and kidneys of three marsupial species (Micoureus paraguayanus, Monodelphis ihering, and Didelphis aurita) as well in two species of bats (Diphylla ecaudata and Anoura caudifer) is of significance because these new hosts could represent an important virus reservoirs.

Conclusions

The analysis of nucleotide sequences of the partial S segment revealed that these genes were more related to the Araraquara virus strains. This work reinforces the importance of studying hantavirus in different animal species and performing a continued surveillance before this virus spreads in new hosts and generated serious problems in public health.

【 授权许可】

   
2012 de Araujo et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Cueto GR, Cavia R, Bellomo C, Padula PJ, Suárez OV: Prevalence of hantavirus infection in wild Rattus norvegicus and R. rattus populations of Buenos Aires City, Argentina. Trop Med Int Health 2008, 13(1):46-51.
• [2]Kim GR, Lee YT, Park C: A new natural reservoir of Hantavirus: isolation of Hantaviruses from lung tissues of bats. Arch Virol 1994, 134:85-95.
• [3]Sumibcay L, Kadjo B, Gu SH, Kang HJ, Lim BK, Cook JA, Song JW, Yanagihara R: Divergent lineage of a novel hantavirus in the banana pipistrelle (Neoromicia nanus) in Côte d’Ivoire. Virol J 2012, 9:34. BioMed Central Full Text
• [4]Araujo J, Pereira A, Nardi MS, Henriques DA, Lautenschalager DA, Dutra LM, Ometto TL, Hurtado RF, Maués F, Nava A, et al.: Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR. Arch Virol 2011, 156:1-6.
• [5]Thomazelli LM, Araujo J, Ferreira CS, Hurtado R, Oliveira DB, Ometto T, Golono M, Sanfilippo L, Demetrio C, Figueiredo ML, et al.: Molecular surveillance of the Newcastle disease virus in domestic and wild birds on the North Eastern Coast and Amazon biome of Brazil. Rev Bras Cienc Avic 2012, 14:1-7.
• [6]Moreli ML, Sousa RLM, Figueiredo LTM: Detection of Brazilian hantavirus by reverse transcription polymerase chain reaction amplification of N gene in patients with hantavirus cardiopulmonary syndrome. Mem Inst Oswaldo Cruz 2004, 99:633-638.
• [7]Swofford DL: PAUP*. phylogenetic analysis using parsimony (*and other methods). Version 4. Sunderland, MA: Sinauer Associates; 2002.
• [8]Felsenstein J: Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985, 39:783-791.
• [9]Suzuki A, Bisordi I, Levis ST, Garcia JT, Pereira LE, Souza RP, Sugahara TKN, Pini NT, Enria DT, Souza LTM: Identifying rodent hantavírus reservoirs, Brazil. Emerg Infect Dis 2004, 10:2127-2134.
• [10]Mills JN, Johnson JM, Ksiazek TG, Ellis BA, Rollin PE, Yates T, Mann MO, Johnson MR, Campbell ML, Miyashiro J, et al.: A survey of Hantavirus antibody in small mammal populations in selected United States national parks. AmJTrop Med Hyg 1998, 58:525-553.
• [11]Jonsson CB, Figueiredo LT, Vapalahti O: A global perspective on hantavirus ecology, epidemiology, and disease. Clin Microbiol Rev 2010, 23:412-441.
• [12]Ramsden C, Holmes EC, Charleston MA: Hantavirus evolution in relation to its rodent and insectivore hosts: no evidence for codivergence. Mol Biol Evol 2009, 26:143-153.
• [13]Jung YT, Kim GR: Genomic characterization of M and S RNA segments of Hantaviruses isolated from bats. Acta Virol 1995, 39:231-233.
• [14]Vasconcelos PFC, Travassos Da Rosa APA, Rodrigues SG, Travassos Da Rosa ES, Dégallier N, Travassos Da Rosa JFS: lnadequate management of natural ecosystem in the Brazilian Amazon region results in the emergence and reemergence of arboviruses. Cad Saude Publica 2001, 17:155-164.
• [15]Bi P, Cameron S, Higgins G, Burrell C: Are humans infected by Hantaviruses in Australia? Internal Med J 2005, 35:672-674.