Bacterial isolates and their antibiotic susceptibility patterns among patients with pus and/or wound discharge at Gondar university hospital

BMC Research Notes

Feleke Moges²  Habtie Tesfa²  Belete Biadgo²  Kasaw Adane²  Tesfaye Nega¹  Getachew Ferede²  Yitayih Wondimeneh²  Dagnachew Muluye² 

[1] Unit of Bacteriology, Gondar University Hospital, P.O. Box 196 Gondar, Ethiopia;School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P.O. Box 196 Gondar, Ethiopia

关键词: Gondar University Hospital;    Wound discharge;    Pus;   

Others  :  1129741
DOI  :  10.1186/1756-0500-7-619

 received in 2014-02-28, accepted in 2014-09-04,  发布年份 2014

【 摘 要 】

Background

In spite of advances in control of infections, wound infections have not completely controlled due to many reasons. The widespread uses of antibiotics, together with the length of time over which they have been available have led to major problems of resistant organisms contributing to morbidity and mortality. This study was aimed to assess bacterial isolates and their drug susceptibility patterns from patients with pus and/or wound discharge.

Methods

A retrospective study was conducted at Gondar University Hospital from all individuals who provide pus and/or wound discharge sample from September, 2009 to August, 2012. Socio-demographic and laboratory results were collected from the University Hospital Microbiology Laboratory unit registration books by using a standard data collection format. Data were analyzed using SPSS version 20 software. P-value ≤ 0.05 was considered statistically significant.

Result

A total of 628 study subjects were included in the study with bacterial isolation rate of 441 (70.2%). Of all, 344 (54.8%) were males. Two hundred eighty two (63.9%) of the isolates were gram positive and 159 (36.1%) were gram negative. About 331/ 441 (75.0%) of the total isolates were Staphylococcus aureus (32.9%), Coagulase Negative staphylococci (14.7%), Streptococcus spp. (11.6%), Escherichia coli (9.5%), Klebsiella spp. (6.3%). The result showed that 66.2% of the isolates were resistant to tetracycline, followed 59.8% for ampicillin, 59.1% for cotrimoxazole, 51.7% for penicillin; least resistant being 6.3% for gentamycin.

Conclusion

High prevalence of bacterial isolates were found; S. aureus being the dominant. Most of the isolates were resistant to many of the antibiotics tested where all isolates of Pseudomonas spp. being resistant to two or more antibiotics. Antibiotic susceptibility test is necessary for effective control of wound infections.

【 授权许可】

   
2014 Muluye et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Howard RJ, Ravitch MM, Steichen FM: Host against Infections. Current Problems in Surgery. New Eng J Med 1980, 12:1823-1830.
• [2]Ogunsola FT, Oduyebo O, Iregbu KC, Coker AD, Adetunji A: A review of nosocomial infections at LUTH; problems and strategies. J Nigerian Infection Control Association 1998, 1:213-217.
• [3]WHO: Surveillance, control and prevention of hospital acquired (nosocomial) infections. Report of an advisory group. 1981. BAC/NIC/81.6
• [4]Thomas KH: Surgical Wound Infection, an Overview. Am J Med 1981, 70:712-718.
• [5]Elmer WK, Stephen DA, William MJ, Schreckenberger PC, Winn WC: Antimicrobial Susceptibility testing in, colour atlas and textbook of Diagnostic Microbiology. 5th edition. Philadelphia: Raven Publisher; 1997:69-120.
• [6]Adebayor OS, Deboye OK, Emiola AR: Wound Infections in two Health Institutions in IIE-Ife, Nigeria: Results of a cohort study. Osto/Wound Manag 2003, 49:52-57.
• [7]Vandepitte A, El-Nageh MM, Stelling JM, Tikhomirov E, Estrela A: WHO Regional Publications, Eastern Mediterranean Series. Volume 15. Alexandria, Egypt: WHO Regional Publications, Eastern Mediterranean Series; 1996.
• [8]National Committee for Clinical Laboratory Standards: Performance standards for antimicrobial susceptibility testing. 7th edition. Wayne, PA: Approved standard M2-A7. National Committee for Clinical Laboratory Standards; 2000.
• [9]Mulu A, Moges F, Tesema B, Kassu A: Pattern and multiple drug resistance of bacterial pathogens isolated from wound infection at University of Gondar teaching hospital, North west Ethiopia. Ethiop Medi J 2006, 44:125-131.
• [10]Malik S, Gupta A, Singh KP, Agarwal J, Singh M: Antibiogram of Aerobic Bacterial Isolates from Post-operative Wound Infections at a Tertiary Care Hospital in India. J Infect Dis Antimicrob Agents 2011, 28:45-51.
• [11]Gebre-Selassie S: Antimicrobial resistance of clinical bacterial isolates in Southern Ethiopia. Ethiopian Med J 2007, 45:363-375.
• [12]Dagnachew M, Yitayih W, Getachew F, Feleke M, Tesfaye N: Bacterial isolates and drug susceptibility patterns of ear discharge from patients with ear infection at Gondar University Hospital, Northwest Ethiopia. BMC Ear Nose Throat Disord 2013, 13:10. BioMed Central Full Text