Colonisation with ESBL-producing and carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant Staphylococcus aureus in a long-term care facility over one year

BMC Infectious Diseases

Dearbháile Morris²  Bernie Austin³  James R Johnson⁴  Akke Vellinga²  Martin Cormican¹  Catherine Ludden² 

[1] Centre for Health from Environment, Ryan Institute, National University of Ireland Galway, Galway, Ireland;Discipline of Bacteriology, School of Medicine, National University of Ireland Galway, Galway, Ireland;St. Brendan’s Home, Community Nursing Unit, Galway, Ireland;VA Medical Center, Minneapolis, MN, USA

关键词: Long-term care facilities;    Meticillin-resistant S. aureus (MRSA);    Vancomycin-resistant enterococci (VRE);    Carbapenemase-producing Enterobacteriaceae (CPE);    Extended-spectrum beta-lactamase (ESBL);   

Others  :  1172105
DOI  :  10.1186/s12879-015-0880-5

 received in 2015-01-05, accepted in 2015-03-12,  发布年份 2015

【 摘 要 】

Background

This study examined colonisation with and characteristics of antimicrobial-resistant organisms among residents of a long-term care facility (LTCF) over one year, including strain persistence and molecular diversity among isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae.

Methods

Sixty-four residents of a LTCF were recruited (51 at baseline, 13 during the year). Data on dependency levels, hospitalisations, and antimicrobial prescribing were collected. Nasal and rectal swabs and catheter urine specimens were examined quarterly, using chromogenic agars, for ESBL-producing Enterobacteriaceae, carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant enterococci (VRE), and meticillin-resistant S. aureus (MRSA). All ESBL-producing E. coli (ESBL-EC) were characterised by pulsed-field gel electrophoresis (PFGE) and PCR to assess for sequence type (ST) ST131, its resistance-associated H30 and H30-Rx subclones, and bla_CTX-M,bla_TEM,bla_SHV, and bla_OXA-1.

Results

The overall number of residents colonised, by organism, was as follows: ESBL-EC, 35 (55%); MRSA, 17 (27%); ESBL-producing K. pneumoniae (ESBL-KP), 5 (8%); VRE, 2 (3%) and CPE, 0 (0%). All 98 ESBL-EC isolates were H30-Rx ST131, with bla_{CTX-M-group 1.} By PFGE, a group of 91 ESBL-EC (from 33 participants) had ≥ 85% similar profiles and resembled UK epidemic strain A/ international pulsotype PFGE812. Sequential ESBL-EC from individual residents were closely related. Six ESBL-KP isolates, from five participants, had bla_{CTX-M-group 1} and by PFGE were closely related. Colonisation with ESBL and MRSA was associated with location within the LTCF and previous exposure to antimicrobials.

Conclusions

Among LTCF residents, colonisation with ESBL-EC and MRSA was common. All ESBL-EC were H30-Rx ST131, consistent with clonal dissemination.

【 授权许可】

   
2015 Ludden et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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