Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage

BMC Complementary and Alternative Medicine

Stephen O Adewole²  Efere M Obuotor¹  Victor O Ukwenya³  David O Adeyemi² 

[1] Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria;Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria;Department of Anatomy, Ekiti State University, Ado Ekiti, Ekiti State, Nigeria

关键词: Antioxidant;    Flavonoids;    Hibiscus sabdariffa;    Diabetes mellitus;    Toxicity;    Liver;   

Others  :  1087127
DOI  :  10.1186/1472-6882-14-277

 received in 2014-04-02, accepted in 2014-07-21,  发布年份 2014

【 摘 要 】

Background

Flavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx was evaluated for its anti-hepatotoxic activities in streptozotocin-induced diabetic Wistar rats.

Methods

Diabetes Mellitus was induced in Wistar rats by a single i.p injection of 80 mg/kg b.w. streptozotocin (STZ) dissolved in 0.1 M citrate buffer (pH 6.3).

Results

The ameliorative effects of the extract on STZ-diabetes induced liver damage was evident from the histopathological analysis and the biochemical parameters evaluated in the serum and liver homogenates. Reduced levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (3.76 ± 0.38 μM, 0.42 ± 0.04 U/L, 41.08 ± 3.04 U/ml, 0.82 ± 0.04 U/L respectively) in the liver of diabetic rats were restored to a near normal level in the Hibiscus sabdariffa -treated rats (6.87 ± 0.51 μM, 0.72 ± 0.06 U/L, 87.92 ± 5.26 U/ml, 1.37 ± 0.06 U/L respectively). Elevated levels of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) in the serum of diabetic rats were also restored in Hibiscus sabdariffa -treated rats. Examination of stained liver sections revealed hepatic fibrosis and excessive glycogen deposition in the diabetic rats. These pathological changes were ameliorated in the extract-treated rats.

Conclusion

The anti-hepatotoxic activity of Hibiscus sabdariffa extract in STZ diabetic rats could be partly related to its antioxidant activity and the presence of flavonnoids.

【 授权许可】

   
2014 Adeyemi et al.; licensee BioMed Central Ltd.

【 图 表 】

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