Predictors of mother and child DNA yields in buccal cell samples collected in pediatric cancer epidemiologic studies: a report from the Children’s Oncology group

BMC Genetics

Logan G Spector¹  Crystal Blommer¹  Erica Langer¹  Anthony J Hooten¹  Julie A Ross¹  Jenny N Poynter² 

[1] Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA;Department of Pediatrics, MMC 715, 420 Delaware St. S.E., Minneapolis, MN 55455, USA

关键词: Pediatric epidemiology;    Buccal cells;    DNA collection;   

Others  :  1086810
DOI  :  10.1186/1471-2156-14-69

 received in 2013-03-19, accepted in 2013-08-03,  发布年份 2013

【 摘 要 】

Background

Collection of high-quality DNA is essential for molecular epidemiology studies. Methods have been evaluated for optimal DNA collection in studies of adults; however, DNA collection in young children poses additional challenges. Here, we have evaluated predictors of DNA quantity in buccal cells collected for population-based studies of infant leukemia (N = 489 mothers and 392 children) and hepatoblastoma (HB; N = 446 mothers and 412 children) conducted through the Children’s Oncology Group. DNA samples were collected by mail using mouthwash (for mothers and some children) and buccal brush (for children) collection kits and quantified using quantitative real-time PCR. Multivariable linear regression models were used to identify predictors of DNA yield.

Results

Median DNA yield was higher for mothers in both studies compared with their children (14 μg vs. <1 μg). Significant predictors of DNA yield in children included case–control status (β = −0.69, 50% reduction, P = 0.01 for case vs. control children), brush collection type, and season of sample collection. Demographic factors were not strong predictors of DNA yield in mothers or children in this analysis.

Conclusions

The association with seasonality suggests that conditions during transport may influence DNA yield. The low yields observed in most children in these studies highlight the importance of developing alternative methods for DNA collection in younger age groups.

【 授权许可】

   
2013 Poynter et al.; licensee BioMed Central Ltd.

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