Microdistribution of the resistance of malaria vectors to deltamethrin in the region of Plateau (southeastern Benin) in preparation for an assessment of the impact of resistance on the effectiveness of Long Lasting Insecticidal Nets (LLINs)

BMC Infectious Diseases

Martin Akogbéto¹  Vincent Corbel²  Achille Massougbodji⁴  Alioun Adéchoubou⁵  Mariam Okè⁵  Frédéric Oké-Agbo¹  Roseric Azondékon⁶  Virgile Gnanguenon¹  Filémon Tokponnon⁵  Lawal Soumanou¹  Innocent Djègbè³  Arthur Sovi¹ 

[1] Centre de Recherche Entomologique de Cotonou, Cotonou, Benin;Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand;International Institute of Tropical Agriculture, Calavi, Bénin;Faculté des Sciences de la Santé de l’Université d’Abomey-Calavi, Cotonou, Benin;Programme Nationale de Lutte contre le Paludisme, Cotonou, Benin;University of Massachusetts Amherst, Amherst, USA

关键词: Benin;    Plateau;    kdr mutation;    Insecticide resistance;    Deltamethrin;    An. gambiae s.s;   

Others  :  1134487
DOI  :  10.1186/1471-2334-14-103

 received in 2013-08-27, accepted in 2014-02-21,  发布年份 2014

【 摘 要 】

Background

This study aims to research two areas, one with a resistant and the other with a susceptible profile of An. gambiae to deltamethrin in the region of Plateau (southern Benin). In each area, eight localities were sought. Both areas were needed for the assessment of the impact of malaria vector resistance to pyrethroids on the effectiveness of Long Lasting Insecticidal Nets (LLINs). The susceptible area of An. gambiae to deltamethrin was used as a control.

Methods

In total, 119 localities in the region of Plateau were screened by sampling An. gambiae s.l larvae. Female mosquitoes resulting from these larvae were exposed to 0.05% deltamethrin following WHO standards. PCR was used to identify species and molecular forms of the dead and alive mosquitoes. Finally, we identified kdr mutations (1014 F and1014S) using the HOLA technique.

Results

Fifty-six out of 119 prospected localities tested positive for Anopheles gambae s.l breeding sites. The results showed that An. gambiae was resistant to deltamethrin in 39 localities and susceptible in only 2 localities; resistance to deltamethrin was suspected in 15 localities. The HOLA technique confirmed the presence of kdr 1014 F mutation and the absence of kdr 1014S mutation. The kdr 1014 F mutation was found in both M and S molecular forms at relatively high frequencies therefore confirming the susceptibility tests.

Conclusion

We were unable to identify the eight susceptible areas due to the overall resistance of An. gambiae to deltamethrin in the region of Plateau. To implement the study, we kept two areas, one with high resistance (R⁺⁺⁺) and the other with low resistance (R⁺) of An. gambiae to deltamethrin.

【 授权许可】

   
2014 Sovi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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