Hepatoprotection with a chloroform extract of Launaea procumbens against CCl4-induced injuries in rats

BMC Complementary and Alternative Medicine

Shumaila Jan³  Bushra Ahmad³  Umbreen Rashid³  Jasia Bokhari³  Mir Sadiq Shah²  Naseer A Shah³  Sumaira Sahreen¹  Mushtaq Ahmed²  Muhammad R Khan³  Rahmat A Khan³ 

[1] Botanical Sciences Division, Pakistan Museum of Natural History, Garden Avenue, Shakarparian, Islamabad, Pakistan;Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology Bannu KPK, Bannu, Pakistan;Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan

关键词: Carbon tetrachloride;    Antioxidant enzymes;    Flavonoids;    Hepatic injuries;    Launaea procumbens;   

Others  :  1232101
DOI  :  10.1186/1472-6882-12-114

 received in 2011-07-11, accepted in 2012-03-21,  发布年份 2012

【 摘 要 】

Background

Launaea procumbens (Asteraceae) is used as a folk medicine to treat hepatic disorders in Pakistan. The effect of a chloroform extract of Launaea procumbens (LPCE) was evaluated against carbon-tetrachloride (CCl₄)-induced liver damage in rats.

Methods

To evaluate the hepatoprotective effects of LPCE, 36 male Sprague–Dawley rats were equally divided into six groups. Animals of group 1 (control) had free access to food and water. Group II received 3 ml/kg of CCl₄ (30% in olive oil v/v) via the intraperitoneal route twice a week for 4 weeks. Group III received 1 ml of silymarin via gavage (100 mg/kg b.w.) after 48 h of CCl₄ treatment whereas groups IV and V were given 1 ml of LPCE (100 and 200 mg/kg b.w., respectively) after 48 h of CCl₄ treatment. Group VI received 1 ml of LPCE (200 mg/kg b.w.) twice a week for 4 weeks. The activities of the antioxidant enzymes catalase, peroxidase (POD), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), glutathione reductase (GSR), glutathione (GSH) and lipid peroxidation (thiobarbituric acid reactive substances (TBARS)) were measured in liver homogenates. DNA damage, argyrophilic nucleolar organizer regions (AgNORs) counts and histopathology were studied in liver samples. Serum was analyzed for various biochemical parameters. Phytochemical composition in LPCE was determined through high-performance liquid chromatography (HPLC).

Results

LPCE inhibited lipid peroxidation, and reduced the activities of aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate dehydrogenase in serum induced by CCl₄. GSH contents were increased as were the activities of antioxidant enzymes (catalase, SOD, GST, GSR, GSH-Px) when altered due to CCl₄ hepatotoxicity. Similarly, absolute liver weight, relative liver weight and the number of hepatic lesions were reduced with co-administration of LPCE. Phyochemical analyses of LPCE indicated that it contained catechin, kaempferol, rutin, hyperoside and myricetin.

Conclusion

These results indicated that Launaea procumbens efficiently protected against the hepatotoxicity induced by CCl₄ in rats, possibly through the antioxidant effects of flavonoids present in LPCE.

【 授权许可】

   
2012 Khan et al.; licensee BioMed Central Ltd.

【 图 表 】

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