Role of extracytoplasmic function sigma factors in biofilm formation of Porphyromonas gingivalis

BMC Oral Health

Hiromasa Hasegawa²  Toshiyuki Kawakami²  Kazuyuki Ishihara⁴  Koji Nakayama¹  Naoya Ohara³  Yukinaga Shibata⁵  Keisuke Nakano²  Tetsuyoshi Inoue³  Masaaki Nakayama³  Eitoyo Kokubu⁴  Kazuko Shibayama⁴  Yuichiro Kikuchi⁴  Satosu Onozawa⁵ 

[1] Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan;Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine, 1780, Hirooka-Gobara, Shiojiri, Japan;Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama, Japan;Department of Microbiology, Tokyo Dental College, 2-1-14, Misaki-cho, Chiyoda-ku, Tokyo, Japan;Department of Oral Microbiology, Matsumoto Dental University, 1780 Gobara, Hiro-oka, Shiojiri, Japan

关键词: Periodontal disease;    Porphyromonas gingivalis;    Biofilm;    Extracytoplasmic function sigma factor;   

Others  :  1090462
DOI  :  10.1186/1472-6831-15-4

 received in 2014-10-02, accepted in 2015-01-09,  发布年份 2015

【 摘 要 】

Background

Porphyromonas gingivalis has been implicated as a major pathogen in the development and progression of chronic periodontitis. P. gingivalis biofilm formation in the subgingival crevice plays an important role in the ability of the bacteria to tolerate stress signals outside the cytoplasmic membrane. Some bacteria use a distinct subfamily of sigma factors to regulate their extracytoplasmic functions (the ECF subfamily). The objective of this study was to determine if P. gingivalis ECF sigma factors affect P. gingivalis biofilm formation.

Methods

To elucidate the role of ECF sigma factors in P. gingivalis, chromosomal mutants carrying a disruption of each ECF sigma factor-encoding gene were constructed. Bacterial growth curves were measured by determining the turbidity of bacterial cultures. The quantity of biofilm growing on plates was evaluated by crystal violet staining.

Results

Comparison of the growth curves of wild-type P. gingivalis strain 33277 and the ECF mutants indicated that the growth rate of the mutants was slightly lower than that of the wild-type strain. The PGN_0274- and PGN_1740-defective mutants had increased biofilm formation compared with the wild-type (p < 0.001); however, the other ECF sigma factor mutants or the complemented strains did not enhance biofilm formation.

Conclusion

These results suggest that PGN_0274 and PGN_1740 play a key role in biofilm formation by P. gingivalis.

【 授权许可】

   
2015 Onozawa et al.; licensee BioMed Central.

【 图 表 】

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