Chagasic patients are able to respond against a viral antigen from influenza virus

BMC Infectious Diseases

Concepción Judith Puerta¹  John Mario González⁸  Manuel Carlos López⁵  Maria C Thomas⁵  Víctor Velasco²  Fernando Rosas²  Zulma Cucunuba⁴  Fanny Guzmán⁷  Adriana Cuéllar³  Natalia Bolaños⁸  Diana Mesa⁶  Paola Lasso⁶ 

[1] Departamento de Microbiología, Facultad de Ciencias, Laboratorio de Parasitología Molecular, Pontificia Universidad Javeriana, Bogotá, Colombia;Fundación Clínica Abood Shaio, Diag. 115A No. 70C – 75, Bogotá, Colombia;Grupo de Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, Carrera 7 No. 43 – 82, Bogotá, Colombia;Grupo de Parasitología, Instituto Nacional de Salud, Avenida Calle 26 No. 51 – 20, Bogotá, Colombia;Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones Científicas (IPBLN-CSIC), Parque Tecnológico de Ciencias de la Salud, Avda. del Conocimiento, s/n.18100, Granada, Spain;Laboratorio de Parasitología Molecular, Pontificia Universidad Javeriana, Carrera 7 No. 43 – 82, Bogotá, Colombia;Núcleo de Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Avenida Universidad 330, Valparaíso, Chile;Grupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, Carrera 1 No. 18A – 10, Bogotá, Colombia

关键词: Nonspecific immune-suppression;    Non-T. cruzi microbial antigen;    Chagas disease;    CD8⁺ T cells;   

Others  :  1159710
DOI  :  10.1186/1471-2334-12-198

 received in 2011-12-02, accepted in 2012-08-13,  发布年份 2012

【 摘 要 】

Background

Trypanosoma cruzi, the etiological agent of Chagas’ disease, is an obligate intracellular parasite which induces a CD8⁺ T cell immune response with secretion of cytokines and release of cytotoxic granules. Although an immune-suppressive effect of T. cruzi on the acute phase of the disease has been described, little is known about the capacity of CD8⁺ T cell from chronic chagasic patients to respond to a non-T. cruzi microbial antigen.

Methods

In the present paper, the frequency, phenotype and the functional activity of the CD8⁺ T cells specific from Flu-MP*, an influenza virus epitope, were determined in 13 chagasic patients and 5 healthy donors.

Results

The results show that Flu-MP* peptide specific CD8⁺ T cells were found with similar frequencies in both groups. In addition, Flu-MP* specific CD8⁺ T cells were distributed in the early or intermediate/late differentiation stages without showing enrichment of a specific sub-population. The mentioned Flu-MP* specific CD8⁺ T cells from chagasic patients were predominately T_EM (CCR7- CD62L-), producing IL-2, IFNγ, CD107a/b and perforin, and did not present significant differences when compared with those from healthy donors.

Conclusions

Our results support the hypothesis that there is no CD8⁺ T cell nonspecific immune-suppression during chronic Chagas disease infection. Nonetheless, other viral antigens must be studied in order to confirm our findings.

【 授权许可】

   
2012 Lasso et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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