Methane-rich saline attenuates ischemia/reperfusion injury of abdominal skin flaps in rats via regulating apoptosis level

BMC Surgery

Xuemei Ma³  Youbin Wang¹  Yifang Liu³  Yunqi Liu³  Jianqiang Hu²  Mingzi Zhang¹  Kexin Song¹ 

[1] Department of Plastic Surgery, Peking Union Medical College Hospital, Beijing, China;Department of Orthopaedics, Qingdao Huangdao District Hospital of Traditional Chinese Medicine, Qingdao, Shandong, China;College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China

关键词: Apoptosis;    Skin flaps;    Ischemia/reperfusion injury;    Methane-rich saline;   

Others  :  1222718
DOI  :  10.1186/s12893-015-0075-4

 received in 2015-03-28, accepted in 2015-07-16,  发布年份 2015

【 摘 要 】

Background

In plastic surgery, skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs. Methane gas has been reported to be a new therapeutic gas for attenuating I/R injury. In this study, we assessed the effects of methane-rich saline (MRS) in regulating apoptosis on skin flap I/R injury.

Methods

Male Sprague–Dawley rats, 6–8 weeks old, were divided randomly into three groups: one sham surgery group (SH) and two surgery groups. After undergoing 6 h of I/R management of an abdominal skin flap, surgery groups were treated with physiological saline (I/R-P) or methane-rich saline (I/R-M). On the 3rd postoperative day, a laser Doppler flowmeter was used to measure flap blood supply, and hematoxylin and eosin (H&E) staining was used to observe morphological changes. TdT-mediated dUTP-X nick end labeling (TUNEL) staining was also used to observe early apoptosis and is presented as the percentage of TUNEL-positive cells. Moreover, pASK-1, pJNK, Bcl-2 and Bax were detected by immunohistochemical technology. Caspase-3 activity was also measured to evaluate the effects of MRS.

Results

Compared to the I/R-P group, the flaps in the I/R-M group presented a larger survival area and better blood perfusion with less inflammatory infiltration and cell apoptosis, a higher expression of Bcl-2, a lower expression of pASK-1, pJNK and Bax, and a lower caspase-3 activity.

Conclusion

According to the results, MRS attenuated I/R injury by regulating apoptosis and has the potential to be applied as a new therapy for improving skin flap survival.

【 授权许可】

   
2015 Song et al.

【 图 表 】

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