Ecotoxicology inside the gut: impact of heavy metals on the mouse microbiome

BMC Clinical Pharmacology

Benoît Foligné¹  Bruno Pot¹  Evie De Brandt³  Peter Vandamme³  Sébastien Massart²  Jérôme Breton¹ 

[1] Bactéries Lactiques & Immunité des Muqueuses, Centre d‘Infection et d’Immunité de Lille, Institut Pasteur de Lille, U1019, UMR 8204, Université Lille Nord de France, 1 rue du Pr Calmette, Lille cedex, BP 245, F-59019, France;DNAVision SA, avenue George Lemaitre 25, Charleroi B-6041, Belgium;Laboratory of Microbiology, Faculty of Sciences, Ledeganckstraat 35, Ghent B-9000, Belgium

关键词: Denaturing gradient gel electrophoresis (DGGE);    Turicibacter;    16S pyrosequencing;    Mice;    Gut microbiota;    Heavy metal exposure;   

Others  :  860468
DOI  :  10.1186/2050-6511-14-62

 received in 2013-10-13, accepted in 2013-12-04,  发布年份 2013

【 摘 要 】

Background

The gut microbiota is critical for intestinal homeostasis. Recent studies have revealed the links between different types of dysbiosis and diseases inside and outside the intestine. Environmental exposure to pollutants (such as heavy metals) can also impair various physiological functions for good health. Here, we studied the impact of up to 8 weeks of oral lead and cadmium ingestion on the composition of the murine intestinal microbiome.

Results

Pyrosequencing of 16S RNA sequences revealed minor but specific changes in bacterial commensal communities (at both family and genus levels) following oral exposure to the heavy metals, with notably low numbers of Lachnospiraceae and high numbers levels of Lactobacillaceae and Erysipelotrichaceacae (mainly due to changes in Turicibacter spp), relative to control animals.

Conclusions

Non-absorbed heavy metals have a direct impact on the gut microbiota. In turn, this may impact the alimentary tract and overall gut homeostasis. Our results may enable more accurate assessment of the risk of intestinal disease associated with heavy metal ingestion.

【 授权许可】

   
2013 Breton et al.; licensee BioMed Central Ltd.

【 图 表 】

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