Characterization of microsatellite markers developed from Prosopis rubriflora and Prosopis ruscifolia (Leguminosae - Mimosoideae), legume species that are used as models for genetic diversity studies in Chaquenian areas under anthropization in South America

BMC Research Notes

Anete P Souza⁴  Ângela LB Sartori¹  Ana MG Azevedo-Tozzi³  Maria I Zucchi²  Fábio M Alves⁴ 

[1]Departamento de Biologia, Centro de Ciências Biológicas e da Saúde (CCBS), Universidade Federal de Mato Grosso do Sul – UFMS, Cidade Universitária s/n, Caixa Postal 549, CEP 79070-900 Campo Grande, MS, Brazil

[2]Pólo Apta Centro Sul, Rod. SP 127 km 30, Piracicaba, SP CP 28, CEP 13400-970, Brazil

[3]Departamento de Biologia Vegetal (BV), Instituto de Biologia, Universidade Estadual de Campinas - UNICAMP, Cidade Universitária Zeferino Vaz, CP 6109, CEP 13083-970 Campinas, SP, Brazil

[4]Centro de Biologia Molecular e Engenharia Genética (CBMEG) - Universidade Estadual de Campinas - UNICAMP, Cidade Universitária Zeferino Vaz, CP 6010, CEP 13083-970 Campinas, SP, Brazil

关键词: Short Tandem Repeats;    Conservation;    Population Genetics;    Chaco;    Pantanal;    Prosopis;   

Others  :  1132453
DOI  :  10.1186/1756-0500-7-375

 received in 2014-01-09, accepted in 2014-06-12,  发布年份 2014

【 摘 要 】

Background

Prosopis rubriflora and Prosopis ruscifolia are important species in the Chaquenian regions of Brazil. Because of the restriction and frequency of their physiognomy, they are excellent models for conservation genetics studies. The use of microsatellite markers (Simple Sequence Repeats, SSRs) has become increasingly important in recent years and has proven to be a powerful tool for both ecological and molecular studies.

Findings

In this study, we present the development and characterization of 10 new markers for P. rubriflora and 13 new markers for P. ruscifolia. The genotyping was performed using 40 P. rubriflora samples and 48 P. ruscifolia samples from the Chaquenian remnants in Brazil. The polymorphism information content (PIC) of the P. rubriflora markers ranged from 0.073 to 0.791, and no null alleles or deviation from Hardy-Weinberg equilibrium (HW) were detected. The PIC values for the P. ruscifolia markers ranged from 0.289 to 0.883, but a departure from HW and null alleles were detected for certain loci; however, this departure may have resulted from anthropic activities, such as the presence of livestock, which is very common in the remnant areas.

Conclusions

In this study, we describe novel SSR polymorphic markers that may be helpful in future genetic studies of P. rubriflora and P. ruscifolia.

【 授权许可】

   
2014 Alves et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Lewis GP, Schrire B, Mackinder B, Lock M: Legumes of the World. Kew: Royal Botanic Gardens; 2005.
• [2]Hassler E: Repert Spec Nov Regni Veg. 1910, 8:552.
• [3]Grisebach A: Plantae Lorentzianae. Abh Kön Ges Wiss Göttingen 1874, 19:130.
• [4]Freyre M, Astrada E, Blasco C, Baigorria C, Rozycki V, Bernardi C: Valores nutricionales de frutos de vinal (Prosopis ruscifolia): consumo animal e humano. Ciência y Tecnologia Alimentaria 2003, 4(1):41-46.
• [5]Freitas TG, Souza CS, Aoki C, Arakaki LMM, Stefanello TH, Sartori ALB, Sigrist MR: Flora of Brazilian humid Chaco: composition and reproductive phenology. Check List 2013, 9(5):973-979.
• [6]Burkart AA: A monograph of the genus Prosopis (Leguminosae Subfam. Mimosoideae). J Arnold Arbore 1976, 57:219-247.
• [7]Prado DE, Gibbs PE, Pott A, Pott VJ: The Chaco-Pantanal transition in southern Mato Grosso, Brazil. In Nature and Dynamics of Forest-Savanna Boundaries. Edited by Furley PA, Proctor J, Ratter JA. London: Chapman and Hall; 1992:451-470.
• [8]Pott A, Pott VJ: Plantas do Pantanal. Corumbá: EMBRAPA; 1994.
• [9]Furtado PP, Guimarães JG, Fonzar BC: Vegetação - As regiões fitoecológicas, sua natureza e seus recursos econômicos. In Projeto RADAMBRASIL. Folha SF 21. Campo Grande; geologia, geomorfologia, pedologia, vegetação e uso potencial da terra. Volume 28. Edited by Divisão de Publicação. Rio de Janeiro: MME/SG/Projeto RADAMBRASIL; 1982:281-336.
• [10]Loureiro RL, Lima JPS, Fonzar BC: Vegetação - As regiões fitoecológicas, sua natureza e seus recursos econômicos. In Projeto RADAMBRASIL. Folha SE 21. Corumbá e parte da folha SE.20; geologia, geomorfologia, pedologia, vegetação e uso potencial da terra. Volume 27. Edited by Divisão de Publicação. Rio de Janeiro: MME/SG/Projeto RADAMBRASIL; 1982:329-366.
• [11]Conner JK, Hartl DL: A Primer of Ecological Genetics. Massachusetts: Sinauer Associates; 2004.
• [12]Ferreira ME, Grattapaglia D: Introdução ao uso de marcadores moleculares em análise genética. Brasília: Embrapa Recursos Genéticos e Biotecnologia; 1998.
• [13]Billotte N, Lagoda PJL, Risterucci AM, Baurens FC: Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 1999, 54(4):277-288.
• [14]Temnykh S, DeClerck G, Lukashova A, Lipovich L: Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): Frequency, length variation, transposon associations, and genetic marker potential. Genome Res 2001, 11:1441-1452.
• [15]Mottura MC, Finkeldey R, Verga AR, Gailing O: Development and characterization of microsatellite markers for Prosopis chilensis and Prosopis flexuosa and cross-species amplification. Mol Ecol Notes 2005, 5:487-489.
• [16]Creste S, Tulmann-Neto A, Figueira A: Detection of single repeat polymorphisms in denaturing polyacrilamide sequencing gels by silver staining. Plant Mol Biol Report 2001, 19:229-306.
• [17]Park SDE: Trypanotolerance in West African Cattle and the Population Genetic Effects of Selection. PhD thesis. University of Dublin, Ireland; 2001
• [18]Raymond M, Rousset F: GENEPOP (Version 1.2): Population Genetics Software for Exact Tests and Ecumenicism. J Hered 1995, 86(3):248-249.
• [19]Chapuis MP, Estoup A: Microsatellite null alleles and estimation of population differentiation. Mol Biol Evol 2007, 24(3):621-631.
• [20]Bessega CF, Pometti CL, Miller JT, Watts R, Saidman BO, Vilardi JC: New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae). Appl Plant Sci 2013, 1(5):1200324.