Effects of stachydrine on norepinephrine-induced neonatal rat cardiac myocytes hypertrophy and intracellular calcium transients

BMC Complementary and Alternative Medicine

Rong Lu¹  Hong-Chang Wei¹  Wei Guo¹  Pei Zhao¹  Yue-Ling Liao¹  Xiao-Li Shan¹  Chen Zhang¹ 

[1] Department of Pathology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

关键词: Stachydrine;    SERCA2a;    Phospholamban;    Cardiac hypertrophy;    Ca²⁺ transients;   

Others  :  1085102
DOI  :  10.1186/1472-6882-14-474

 received in 2014-08-04, accepted in 2014-11-27,  发布年份 2014

【 摘 要 】

Background

Leonurus heterophyllus sweet has been suggested to have cardioprotective effects against heart diseases, including ischemic diseases and ventricular remodeling. However, the active ingredients of the herb and the underlying mechanisms are poorly understood. The aim of the present study was to investigate the effects of stachydrine (STA), a major constituent of Leonurus heterophyllus sweet, on norepinephrine (NE) induced hypertrophy and the changes of calcium transients in neonatal rat cardiomyocytes.

Methods

Ventricular myocytes from 1-day-old Wistar rats were isolated and cultured in DMEM/F12 with 1 μmol/L norepinephrine in the presence or absence of 10 μmol/L STA for 72 h. Cardiomyocytes hypertrophy was evaluated by cell surface area, total protein/DNA content, β/α-MHC mRNA ratio. While calcium handling function was evaluated by Ca²⁺-transient amplitude and decay, SERCA2a activity and expression, PLN expression and phosphorylation. β1-adrenergic receptor system activation was evaluated by the content of cAMP and the activation of PKA.

Results

NE treatment increases the cell surface area, protein synthesis, the expression level of β-MHC and β/α-MHC ratio. These effects were attenuated by STA. NE-induced hypertrophy was associated with increased Ca²⁺-transient amplitude, accelerated decay of the Ca²⁺-transient, increased phospholamban expression, hyper-phosphorylation at both the serine-16 and threonine-17 residues, increased intracellular cAMP level, and PKA overactivation. All of which were significantly inhibited by STA.

Conclusion

These data suggest that STA attenuates norepinephrine-induced cardiomyocyte hypertrophy and has potential protective effects against β-adrenergic receptor induced Ca²⁺ mishandling.

【 授权许可】

   
2014 Zhang et al.; licensee BioMed Central.

【 图 表 】

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