Global liver gene expression differences in Nelore steers with divergent residual feed intake phenotypes

BMC Genomics

Luciana CA Regitano⁴  Jeremy F Taylor²  Mauricio A Mudadu⁴  Adhemar Zerlotini-Neto³  Dante PD Lanna⁶  Amália S Chaves⁶  Rymer R Tullio⁴  Gerson B Mourão⁶  Marcela M Souza¹  Priscila SN Oliveira¹  Kamila O Rosa⁵  Robert D Schnabel²  Jared E Decker²  Luiz L Coutinho⁶  Polyana C Tizioto² 

[1]Department of Genetics and Evolution, Federal University of Sao Carlos, São Carlos, SP, Brazil

[2]Division of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA

[3]Embrapa Agricultural Informatics, Campinas, SP, Brazil

[4]Embrapa Southeast Livestock, São Carlos, SP, Brazil

[5]Department of Animal Science, State University of Sao Paulo, Jaboticabal, SP, Brazil

[6]Department of Animal Science, University of São Paulo/ESALQ, Piracicaba, São Paulo, Brazil

关键词: Transcriptomics;    Feed efficiency;    RFI;    Bos indicus;   

Others  :  1149194
DOI  :  10.1186/s12864-015-1464-x

 received in 2014-10-15, accepted in 2015-03-13,  发布年份 2015

【 摘 要 】

Background

Efficiency of feed utilization is important for animal production because it can reduce greenhouse gas emissions and improve industry profitability. However, the genetic basis of feed utilization in livestock remains poorly understood. Recent developments in molecular genetics, such as platforms for genome-wide genotyping and sequencing, provide an opportunity to identify genes and pathways that influence production traits. It is known that transcriptional networks influence feed efficiency-related traits such as growth and energy balance. This study sought to identify differentially expressed genes in animals genetically divergent for Residual Feed Intake (RFI), using RNA sequencing methodology (RNA-seq) to obtain information from genome-wide expression profiles in the liver tissues of Nelore cattle.

Results

Differential gene expression analysis between high Residual Feed Intake (HRFI, inefficient) and low Residual Feed Intake (LRFI, efficient) groups was performed to provide insights into the molecular mechanisms that underlie feed efficiency-related traits in beef cattle. A total of 112 annotated genes were identified as being differentially expressed between animals with divergent RFI phenotypes. These genes are involved in ion transport and metal ion binding; act as membrane or transmembrane proteins; and belong to gene clusters that are likely related to the transport and catalysis of molecules through the cell membrane and essential mechanisms of nutrient absorption. Genes with functions in cellular signaling, growth and proliferation, cell death and survival were also differentially expressed. Among the over-represented pathways were drug or xenobiotic metabolism, complement and coagulation cascades, NRF2-mediated oxidative stress, melatonin degradation and glutathione metabolism.

Conclusions

Our data provide new insights and perspectives on the genetic basis of feed efficiency in cattle. Some previously identified mechanisms were supported and new pathways controlling feed efficiency in Nelore cattle were discovered. We potentially identified genes and pathways that play key roles in hepatic metabolic adaptations to oxidative stress such as those involved in antioxidant mechanisms. These results improve our understanding of the metabolic mechanisms underlying feed efficiency in beef cattle and will help develop strategies for selection towards the desired phenotype.

【 授权许可】

   
2015 Tizioto et al.; licensee Biomed Central.

【 图 表 】

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