BMC Musculoskeletal Disorders | |
The efficacy of a lysine-based dendritic hydrogel does not differ from those of commercially available tissue sealants and adhesives: an ex vivo study | |
Ara Nazarian3  Edward K Rodríguez2  Mark W Grinstaff1  Lorenzo Anez-Bustillos3  Cynthia Ghobril1  Juan C Villa-Camacho3  | |
[1] Departments of Biomedical Engineering and Chemistry, Boston University, Boston, MA, USA;Carl J Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA;Center for Advanced Orthopaedic Studies, Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA | |
关键词: Dendron; Adhesive; Sealant; Hydrogel; Trauma; Hemostasis; | |
Others : 1229769 DOI : 10.1186/s12891-015-0573-7 |
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received in 2014-11-18, accepted in 2015-04-29, 发布年份 2015 | |
【 摘 要 】
Background
Hemostatic agents, tissue adhesives and sealants may contribute to a reduction in hemorrhage-associated morbidity and mortality. Towards this end, we have recently developed a lysine-based dendritic hydrogel (PEG-LysNH2) that can potentially be used in the management of severe trauma and/or intraoperative bleeding. As a first step in demonstrating the potential utility of this approach, our objective was to ascertain the ability of the PEG-LysNH2 to adhere to and seal injured tissues, as well as to maintain the seal under physiological conditions.
Methods
The efficacy of the PEG-LysNH2 in sealing injured tissues was evaluated using an ex-vivo pressure testing system. A 2.5 mm incision was made on intact ex-vivo tissues and then sealed with the PEG-LysNH2. Application of the PEG-LysNH2 was followed by 1) step-wise pressure increase to a maximum of 250 mmHg and 2) fluctuating pressures, between 100–180 mmHg with a rate of 3 Hz, over a 24-hour period. The performance of the PEG-LysNH2 was compared to those of commercially available sealants and adhesives.
Results
During gradual pressure increase, mean pressures at 30 seconds (P30) ranged between 206.36 - 220.17 mmHg for the sealants, and they were greater than control and suture groups (p < 0.01 and p = 0.013, respectively). Additionally, all products held under fluctuating pressures: mean pressures ranged between 135.20 - 160.09 mmHg, and there were no differences observed between groups (p = 0.96).
Conclusions
The efficacy of the PEG-LysNH2 was significantly superior to conventional injury repair methods (sutures) and did not differ from those of commercially available products when sealing small incisions.
【 授权许可】
2015 Villa-Camacho et al.; licensee BioMed Central.
【 预 览 】
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20151031024337947.pdf | 1461KB | download | |
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Figure 1. | 17KB | Image | download |
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