The efficacy of a lysine-based dendritic hydrogel does not differ from those of commercially available tissue sealants and adhesives: an ex vivo study

BMC Musculoskeletal Disorders

Ara Nazarian³  Edward K Rodríguez²  Mark W Grinstaff¹  Lorenzo Anez-Bustillos³  Cynthia Ghobril¹  Juan C Villa-Camacho³ 

[1] Departments of Biomedical Engineering and Chemistry, Boston University, Boston, MA, USA;Carl J Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA;Center for Advanced Orthopaedic Studies, Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

关键词: Dendron;    Adhesive;    Sealant;    Hydrogel;    Trauma;    Hemostasis;   

Others  :  1229769
DOI  :  10.1186/s12891-015-0573-7

 received in 2014-11-18, accepted in 2015-04-29,  发布年份 2015

【 摘 要 】

Background

Hemostatic agents, tissue adhesives and sealants may contribute to a reduction in hemorrhage-associated morbidity and mortality. Towards this end, we have recently developed a lysine-based dendritic hydrogel (PEG-LysNH₂) that can potentially be used in the management of severe trauma and/or intraoperative bleeding. As a first step in demonstrating the potential utility of this approach, our objective was to ascertain the ability of the PEG-LysNH₂ to adhere to and seal injured tissues, as well as to maintain the seal under physiological conditions.

Methods

The efficacy of the PEG-LysNH₂ in sealing injured tissues was evaluated using an ex-vivo pressure testing system. A 2.5 mm incision was made on intact ex-vivo tissues and then sealed with the PEG-LysNH₂. Application of the PEG-LysNH₂ was followed by 1) step-wise pressure increase to a maximum of 250 mmHg and 2) fluctuating pressures, between 100–180 mmHg with a rate of 3 Hz, over a 24-hour period. The performance of the PEG-LysNH₂ was compared to those of commercially available sealants and adhesives.

Results

During gradual pressure increase, mean pressures at 30 seconds (P₃₀) ranged between 206.36 - 220.17 mmHg for the sealants, and they were greater than control and suture groups (p < 0.01 and p = 0.013, respectively). Additionally, all products held under fluctuating pressures: mean pressures ranged between 135.20 - 160.09 mmHg, and there were no differences observed between groups (p = 0.96).

Conclusions

The efficacy of the PEG-LysNH₂ was significantly superior to conventional injury repair methods (sutures) and did not differ from those of commercially available products when sealing small incisions.

【 授权许可】

   
2015 Villa-Camacho et al.; licensee BioMed Central.

【 图 表 】

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