Association between single-nucleotide polymorphisms and early spontaneous hepatitis B virus e antigen seroconversion in children

BMC Research Notes

Tomoo Fujisawa²  Tsuyoshi Sogo²  Tomoyuki Tsunoda²  Ayano Inui²  Jun Murakami¹  Haruki Komatsu² 

[1] Division of Pediatrics and Perinatology, Faculty of Medicine, Tottori University, Yonago, Japan;Division of Hepatology and Gastroenterology, Department of Pediatrics, Eastern Yokohama Hospital, Yokohama, Japan

关键词: HLA-DP;    Children;    Seroconversion;    HBeAg;    IL10;    IL28B;    Human leukocyte antigen;    Single-nucleotide polymorphism;    Hepatitis B virus;   

Others  :  1125673
DOI  :  10.1186/1756-0500-7-789

 received in 2014-06-16, accepted in 2014-10-24,  发布年份 2014

【 摘 要 】

Background

The disease progression following hepatitis B virus (HBV) infection is associated with single-nucleotide polymorphisms (SNPs). However, the role of SNPs in chronic HBV infection in children remains unclear. Here, we investigate the association between SNPs and early spontaneous hepatitis B e antigen (HBeAg) seroconversion in children with chronic hepatitis B infection.

Methods

This was a retrospective cohort study. We genotyped seven SNPs in the following genes, interleukin (IL)-10 (rs1800871 and rs1800872), human leukocyte antigen (HLA)-DPA1 (rs3077), HLA-DPB1 (rs9277535), HLA-DQB2 (rs7453920), HLA-DQB1 (rs2856718), and IL28B (rs8099917), in patients with chronic HBV infection using PCR and sequencing. These variants were analyzed for an association with early HBeAg seroconversion in children.

Results

Of 225 Japanese patients with chronic hepatitis B virus infection (male/female: 105/120, median age at initial visit: 6 years; range 0–44 years), 52 achieved spontaneous HBeAg seroconversion at the age of 10 years or younger (G1: early seroconversion group), and 57 did not achieve spontaneous HBeAg seroconversion under the age of 20 years (G2: late or no seroconversion group). Of the seven SNPs, only the HLA-DPA1 SNP displayed a low p-value (P = 0.070), but not significant, to have early HBeAg seroconversion in the dominant model and in the allele model (P =  0.073) using the chi-square test. The association study found a low p-value, but not significant, to have early HBeAg seroconversion in the dominant model for HLA-DPA1 (genotype TC + TT vs. CC, P = 0.070, odds ratio: 2.016, 95% confidence interval: 0.940-4.323) using a logistic regression model.

Conclusion

Although the HLA-DPA1 SNP did not show a statistically significant association with early HBeAg seroconversion in this study, the HLA-DPA1 SNP might increase the likelihood of achieving early spontaneous HBeAg seroconversion in children.

【 授权许可】

   
2014 Komatsu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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