Antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens

BMC Microbiology

Chrysanthi Paranavitana¹  Sneha Patel¹  Ruchi Patel¹  Xiaorong Feng¹  Karthik Sambanthamoorthy¹ 

[1] Department of Wound Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA

关键词: Lactobacillus rhamnosus;    Lactobacillus jensenii;    Antimicrobial;    Biosurfactant;    Anti-biofilm;    Anti-adhesive;   

Others  :  1140587
DOI  :  10.1186/1471-2180-14-197

 received in 2014-04-01, accepted in 2014-06-27,  发布年份 2014

【 摘 要 】

Background

Biosurfactants (BS) are amphiphilic compounds produced by microbes, either on the cell surface or secreted extracellularly. BS exhibit strong antimicrobial and anti-adhesive properties, making them good candidates for applications used to combat infections. In this study, our goal was to assess the in vitro antimicrobial, anti-adhesive and anti-biofilm abilities of BS produced by Lactobacillus jensenii and Lactobacillus rhamnosus against clinical Multidrug Resistant (MDR) strains of Acinetobacter baumannii, Escherichia coli, and Staphylococcus aureus (MRSA). Cell-bound BS from both L. jensenii and L. rhamnosus were extracted and isolated. The surface activities of crude BS samples were evaluated using an oil spreading assay. The antimicrobial, anti-adhesive and anti-biofilm activities of both BS against the above mentioned MDR pathogens were determined.

Results

Surface activities for both BS ranged from 6.25 to 25 mg/ml with clear zones observed between 7 and 11 cm. BS of both L. jensenii and L. rhamnosus showed antimicrobial activities against A. baumannii, E. coli and S. aureus at 25-50 mg/ml. Anti-adhesive and anti-biofilm activities were also observed for the aforementioned pathogens between 25 and 50 mg/ml. Finally, analysis by electron microscope indicated that the BS caused membrane damage for A. baumannii and pronounced cell wall damage in S. aureus.

Conclusion

Our results indicate that BS isolated from two Lactobacilli strains has antibacterial properties against MDR strains of A. baumannii, E. coli and MRSA. Both BS also displayed anti-adhesive and anti-biofilm abilities against A. baumannii, E. coli and S. aureus. Together, these capabilities may open up possibilities for BS as an alternative therapeutic approach for the prevention and/or treatment of hospital-acquired infections.

【 授权许可】

   
2014 Sambanthamoorthy et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Van Hamme JD, Singh A, Ward OP: Physiological aspects. Part 1 in a series of papers devoted to surfactants in microbiology and biotechnology. Biotechnol Adv 2006, 24(6):604-620.
• [2]Cameotra SS, Makkar RS, Kaur J, Mehta S: Synthesis of biosurfactants and their advantages to microorganisms and mankind. Biosurfaces 2010, 672:261-280.
• [3]Singh P, Cameotra SS: Potential applications of microbial surfactants in biomedical sciences. Trends Biotechnol 2004, 22(3):142-146.
• [4]Ahimou F, Jacques P, Deleu M: Surfactin and iturin A effects on bacillus subtilis surface hydrophobicity. Enzyme Microb Technol 2000, 27(10):749-754.
• [5]Arutchelvi JI, Bhaduri S, Uppara PV, Doble M: Mannosylerythritol lipids: a review. J Ind Microbiol Biotechnol 2008, 35(12):1559-1570.
• [6]Benincasa M, Abalos A, Oliveira I, Manresa A: Chemical structure, surface properties and biological activities of the biosurfactant produced by Pseudomonas aeruginosa LBI from soapstock. Antonie Van Leeuwenhoek 2004, 85(1):1-8.
• [7]Rodrigues L, van der Mei H, Banat IM, Teixeira J, Oliveira R: Inhibition of microbial adhesion to silicone rubber treated with biosurfactant from Streptococcus thermophilus A. FEMS Immunol Med Microbiol 2006, 46(1):107-112.
• [8]Rodrigues L, van der Mei H, Teixeira JA, Oliveira R: Biosurfactant from Lactococcus lactis 53 inhibits microbial adhesion on silicone rubber. Appl Microbiol Biotechnol 2004, 66(3):306-311.
• [9]Rodrigues L, van der Mei HC, Teixeira J, Oliveira R: Influence of biosurfactants from probiotic bacteria on formation of biofilms on voice prostheses. Appl Environ Microbiol 2004, 70(7):4408-4410.
• [10]Velraeds MMC, van der Mei HC, Reid G, Busscher HJ: Physicochemical and biochemical characterization of biosurfactants released by Lactobacillus strains. Colloids Surf B Biointerfaces 1996, 8(1–2):51-61.
• [11]Rodrigues L, Banat IM, Teixeira J, Oliveira R: Biosurfactants: potential applications in medicine. J Antimicrob Chemother 2006, 57(4):609-618.
• [12]Velraeds MM, van der Mei HC, Reid G, Busscher HJ: Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates. Appl Environ Microbiol 1996, 62(6):1958-1963.
• [13]Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM: Microbial biofilms. Annu Rev Microbiol 1995, 49:711-745.
• [14]Anderl JN, Franklin MJ, Stewart PS: Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrob Agents Chemother 2000, 44(7):1818-1824.
• [15]Fux CA, Costerton JW, Stewart PS, Stoodley P: Survival strategies of infectious biofilms. Trends Microbiol 2005, 13(1):34-40.
• [16]Fusetani N: Biofouling and antifouling. Nat Prod Rep 2004, 21(1):94-104.
• [17]Qian PY, Xu Y, Fusetani N: Natural products as antifouling compounds: recent progress and future perspectives. Biofouling 2010, 26(2):223-234.
• [18]Tahmourespour A, Salehi R, Kermanshahi RK, Eslami G: The anti-biofouling effect of Lactobacillus fermentum-derived biosurfactant against Streptococcus mutans. Biofouling 2011, 27(4):385-392.
• [19]Dusane DH, Nancharaiah YV, Zinjarde SS, Venugopalan VP: Rhamnolipid mediated disruption of marine Bacillus pumilus biofilms. Colloids Surf B Biointerfaces 2010, 81(1):242-248.
• [20]Dusane DH, Rajput JK, Kumar AR, Nancharaiah YV, Venugopalan VP, Zinjarde SS: Disruption of fungal and bacterial biofilms by lauroyl glucose. Lett Appl Microbiol 2008, 47(5):374-379.
• [21]Rivardo F, Turner RJ, Allegrone G, Ceri H, Martinotti MG: Anti-adhesion activity of two biosurfactants produced by Bacillus spp. prevents biofilm formation of human bacterial pathogens. Appl Microbiol Biotechnol 2009, 83(3):541-553.
• [22]Irie Y, O’Toole GA, Yuk MH: Pseudomonas aeruginosa rhamnolipids disperse Bordetella bronchiseptica biofilms. FEMS Microbiol Lett 2005, 250(2):237-243.
• [23]Rasmussen TB, Givskov M: Quorum-sensing inhibitors as anti-pathogenic drugs. Int J Med Microbiol 2006, 296(2–3):149-161.
• [24]Valle J, Da Re S, Henry N, Fontaine T, Balestrino D, Latour-Lambert P, Ghigo JM: Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide. Proc Natl Acad Sci U S A 2006, 103(33):12558-12563.
• [25]Walencka E, Rozalska S, Sadowska B, Rozalska B: The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol 2008, 53(1):61-66.
• [26]Busscher HJ, van Hoogmoed CG, Geertsema-Doornbusch GI, van der Kuijl-Booij M, van der Mei HC: Streptococcus thermophilus and its biosurfactants inhibit adhesion by Candida spp. on silicone rubber. Appl Environ Microbiol 1997, 63(10):3810-3817.
• [27]Gan BS, Kim J, Reid G, Cadieux P, Howard JC: Lactobacillus fermentum RC-14 inhibits Staphylococcus aureus infection of surgical implants in rats. J Infect Dis 2002, 185(9):1369-1372.
• [28]Rodrigues LR, Teixeira JA, van der Mei HC, Oliveira R: Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A. Colloids Surf B Biointerfaces 2006, 53(1):105-112.
• [29]Rodrigues LR, Teixeira JA, van der Mei HC, Oliveira R: Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53. Colloids Surf B Biointerfaces 2006, 49(1):79-86.
• [30]Singh A, Van Hamme JD, Ward OP: Surfactants in microbiology and biotechnology: Part 2. Application aspects. Biotechnol Adv 2007, 25(1):99-121.
• [31]Satpute S, Bhawsar BD, Dhakephalkar PK, Chopade BA: Assessment of different screening methods for selecting biosurfactant producing marine bacteria. IJMS 2008, 37(3):243-250.
• [32]Walter V, Syldatk C, Hausmann R: Screening concepts for the isolation of biosurfactant producing microorganisms. Adv Exp Med Biol 2010, 672:1-13.
• [33]Gudina EJ, Rocha V, Teixeira JA, Rodrigues LR: Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20. Lett Appl Microbiol 2010, 50(4):419-424.
• [34]Sriram MI, Kalishwaralal K, Deepak V, Gracerosepat R, Srisakthi K, Gurunathan S: Biofilm inhibition and antimicrobial action of lipopeptide biosurfactant produced by heavy metal tolerant strain Bacillus cereus NK1. Colloids Surf B Biointerfaces 2011, 85(2):174-181.
• [35]Mukherjee S, Das P, Sivapathasekaran C, Sen R: Antimicrobial biosurfactants from marine Bacillus circulans: extracellular synthesis and purification. Lett Appl Microbiol 2009, 48(3):281-288.
• [36]Nitschke M, Costa SGVAO: Biosurfactants in food industry. Trends Food Sci Technol 2007, 18(5):252-259.
• [37]Falagas ME, Makris GC: Probiotic bacteria and biosurfactants for nosocomial infection control: a hypothesis. J Hosp Infect 2009, 71(4):301-306.
• [38]Sambanthamoorthy K, Sloup RE, Parashar V, Smith JM, Kim EE, Semmelhack MF, Neiditch MB, Waters CM: Identification of small molecules that antagonize diguanylate cyclase enzymes to inhibit biofilm formation. Antimicrob Agents Chemother 2012, 56(10):5202-5211.
• [39]Sambanthamoorthy K, Schwartz A, Nagarajan V, Elasri MO: The role of msa in staphylococcus aureus biofilm formation. BMC Microbiol 2008, 8:221.
• [40]Feng X, Sambanthamoorthy K, Palys T, Paranavitana C: The human antimicrobial peptide LL-37 and its fragments possess both antimicrobial and antibiofilm activities against multidrug-resistant Acinetobacter baumannii. Peptides 2013, 49:131-137.