Effect of a mouthrinse containing rice peptide CL(14-25) on early dental plaque regrowth: a randomized crossover pilot study

BMC Research Notes

Atsushi Saito³  Masayuki Taniguchi⁴  Eiichi Saitoh¹  Hiroki Sugito²  Eiichi Suzuki⁶  Koki Ota³  Daichi Kita⁶  Kentaro Imamura⁶  Tetsuo Kato⁵  Saori Takayama⁶ 

[1] Graduate School of Technology, Niigata Institute of Technology, Niigata, Japan;Department of Endodontics and Clinical Cariology, Tokyo Dental College, Tokyo, Japan;Oral Health Science Center, Tokyo Dental College, Tokyo, Japan;Center for Transdisciplinary Research, Niigata University, Niigata, Japan;Laboratory of Chemistry, Tokyo Dental College, Tokyo, Japan;Department of Periodontology, Tokyo Dental College, Tokyo, Japan

关键词: Biofilm;    Plaque;    Antimicrobial agents;    Rice peptide;    Mouthrinse;    Periodontal disease;   

Others  :  1229877
DOI  :  10.1186/s13104-015-1527-8

 received in 2015-05-22, accepted in 2015-09-25,  发布年份 2015

【 摘 要 】

Background

We aimed to evaluate clinically the effect of mouthrinse containing a rice peptide on early dental plaque regrowth.

Methods

The study was designed as a double-masked, two-group crossover randomized pilot trial, involving 10 periodontally healthy volunteers. After receiving a professional tooth cleaning at baseline, over the next 3 days each participant refrained from all oral hygiene measures and had two daily rinses with 20 ml of the test mouthrinse containing 0.4 % rice peptide CL(14-25) or placebo rinse. At the end of each experimental period, plaque score was assessed using the modified Volpe’s method, and the participants filled out a questionnaire. Each participant underwent a 7-day washout period followed by a second allocation. The plaque score was the primary outcome of the study and participant perception was the secondary outcome.

Results

No adverse effects were observed in the participants during the study. Clinically, the mean plaque score of the examined teeth was significantly lower in the test group (2.44 ± 0.74, CI: 1.91–2.96) than the placebo group (2.65 ± 0.63, CI: 2.20–3.10) (P < 0.05). When analyzed according to the type of teeth, a significantly lower score of the premolars/molars was observed in the test group (2.39 ± 0.68, CI: 2.08–2.71) than that in the placebo group (2.66 ± 0.58, CI: 2.39–2.93) (P < 0.05).

Conclusions

The mouthrinse containing 0.4 % rice peptide CL(14-25) was effective in reducing the early regrowth of dental plaque. However, clinical relevance of this efficacy needs to be validated in a future large-scale study.

Trial registration: UMIN Clinical Trials Registry (UMIN-CTR) R000014000. Date of formal registration: November 1, 2013.

【 授权许可】

   
2015 Takayama et al.

【 图 表 】

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【 参考文献 】

• [1]Pihlstrom BL, Michalowicz BS, Johnson NW: Periodontal diseases. Lancet 2005, 366:1809-1820.
• [2]Marsh PD: Controlling the oral biofilm with antimicrobials. J Dent 2010, 38(Suppl 1):S11-S15.
• [3]Baehni PC, Takeuchi Y: Anti-plaque agents in the prevention of biofilm-associated oral diseases. Oral Dis 2003, 9(Suppl 1):23-29.
• [4]Brading MG, Marsh PD: The oral environment: the challenge for antimicrobials in oral care products. Int Dent J 2003, 53(Suppl 1):353-362.
• [5]Serrano J, Escribano M, Roldán S, Martín C, Herrera D: Efficacy of adjunctive anti-plaque chemical agents in managing gingivitis: a systematic review and meta-analysis. J Clin Periodontol 2015, 42:S106-S138.
• [6]Gunsolley JC: Clinical efficacy of antimicrobial mouthrinses. J Dent 2010, 38(Suppl 1):S6-S10.
• [7]Van Leeuwen MPC, Slot DE, Van der Weijden GA: Essential oils compared to chlorhexidine with respect to plaque and parameters of gingival inflammation: a systematic review. J Periodontol. 2011, 82:174-194.
• [8]Van Strydonck DAC, Slot DE, Van der Velden U, Van der Weijden F: Effect of a chlorhexidine mouthrinse on plaque, gingival inflammation and staining in gingivitis patients: a systematic review. J Clin Periodontol 2012, 39:1042-1055.
• [9]Brown KL, Hancock RE: Cationic host defense (antimicrobial) peptides. Curr Opin Immunol 2006, 18:24-30.
• [10]Taiyoji M, Shitomi Y, Taniguchi M, Saitoh E, Ohtsubo S: Identification of proteinaceous inhibitors of a cysteine proteinase (an Arg-specific gingipain) from Porphyromonas gingivalis in rice grain, using targeted-proteomics approaches. J Proteome Res 2009, 8:5165-5174.
• [11]Takei N, Takahashi N, Takayanagi T, Ikeda A, Hashimoto K, Takagi M, et al.: Antimicrobial activity and mechanism of action of a novel cationic alpha-helical dodecapeptide, a partial sequence of cyanate lyase from rice. Peptides 2013, 42:55-62.
• [12]Taniguchi M, Matsuhashi Y, Abe TK, Ishiyama Y, Saitoh E, Kato T, et al.: Contribution of cationic amino acids toward the inhibition of Arg-specific cysteine proteinase (Arg-gingipain) by the antimicrobial dodecapeptide, CL(14-25), from rice protein. Biopolymers 2014, 102:379-389.
• [13]Takayama S, Hashimoto K, Kokubu E, Taniguchi M, Tajima K, Ochiai A, et al. Inhibitory effects of a novel catinonic dodecapeptide [CL(14-25)] derived from cyanate lyase of rice on endotoxic activities of LPSs from Escherichia coli and periodontopathic Aggregatibacter actinomycetemcomitans. Microb Pathog. 2015. doi:10.1016/j.micpath.2015.08.011.
• [14]Volpe AR, Manhold JH, Hazen SP: In vivo calculus assessement. I. A method and its examiner reproducibility. J Periodontol 1965, 36:292-298.
• [15]Manhold JH, Volpe AR, Parker L, Adams SH: In vivo calculus assessment II. A comparison of scoring techiniques. J Periodontol 1965, 36:299-304.
• [16]Suzuki K, Sueda T, Asa R, Toyoda M, Kinoshita S: The effect of different types of toothbrushes on oral hygiene. J Dent Health. 1971, 20:223-230.
• [17]Rams TE, Oler J, Listgarten MA, Slots J: Utility of Ramfjord index teeth to assess periodontal disease progression in longitudinal studies. J Clin Periodontol 1993, 20:147-150.
• [18]Tsunoda M, Sawa M, Moriyama T, Nakazaki T, Nakazaki T, Ota Y, et al.: The effect of dental rinse of cetylpyridinium chloride on bacterial plaque. Jpn J Conserv Dent. 1991, 34:1722-1728.
• [19]Addy M, Willis L, Moran J: Effect of toothpaste rinses compared with chlorhexidine on plaque formation during a 4-day period. J Clin Periodontol 1983, 10:89-99.
• [20]Marchetti E, Mummolo S, Di Mattia J, Casalena F, Di Martino S, Mattei A, et al.: Efficacy of essential oil mouthwash with and without alcohol: a 3-day plaque accumulation model. Trials. 2011, 12:262. BioMed Central Full Text
• [21]Takayama S, Saitoh E, Kimizuka R, Yamada S, Kato T: Effect of eel galectin AJL-1 on periodontopathic bacterial biofilm formation and their lipopolysaccharide-mediated inflammatory cytokine induction. Int J Antimicrob Agents 2009, 34:355-359.
• [22]Aparna S, Srirangarajan S, Malgi V, Setlur KP, Shashidhar R, Setty S, et al.: A comparative evaluation of the antibacterial efficacy of honey in vitro and antiplaque efficacy in a 4-day plaque regrowth model in vivo: preliminary results. J Periodontol 2012, 83:1116-1121.
• [23]Tipton DA, Babu JP, Dabbous M: Effects of cranberry components on human aggressive periodontitis gingival fibroblasts. J Periodontal Res 2013, 48:433-442.
• [24]González OA, Escamilla C, Danaher RJ, Dai J, Ebersole JL, Mumper RJ, et al.: Antibacterial effects of blackberry extract target periodontopathogens. J Periodontal Res 2013, 48:80-86.
• [25]Chen Y, Wong RW, McGrath C, Hagg U, Seneviratne CJ: Natural compounds containing mouthrinses in the management of dental plaque and gingivitis: a systematic review. Clin Oral Investig. 2014, 18:1-16.
• [26]Moran J, Addy M, Newcombe R: A 4-day plaque regrowth study comparing an essential oil mouthrinse with a triclosan mouthrinse. J Clin Periodontol 1997, 24(Pt 1):636-639.
• [27]Moran J, Addy M, Jackson R, Newcombe RG: Comparative effects of quaternary ammonium mouthrinses on 4-day plaque regrowth. J Clin Periodontol 2000, 27:37-40.
• [28]Rosin M, Welk A, Kocher T, Majic-Todt A, Kramer A, Pitten FA: The effect of a polyhexamethylene biguanide mouthrinse compared to an essential oil rinse and a chlorhexidine rinse on bacterial counts and 4-day plaque regrowth. J Clin Periodontol 2002, 29:392-399.
• [29]Moran J, Addy M, Roberts S: A comparison of natural product, triclosan and chlorhexidine mouthrinses on 4-day plaque regrowth. J Clin Periodontol 1992, 19:578-582.
• [30]Riep BG, Bernimoulin JP, Barnett ML: Comparative antiplaque effectiveness of an essential oil and an amine fluoride/stannous fluoride mouthrinse. J Clin Periodontol 1999, 26:164-168.
• [31]Nakagawa T, Isobe S, Ikegami A, Hayashi M, Shibukawa Y, Sumii H, et al.: Effectiveness of electric toothbrush for plaque removal—differences from manual toothbrush in brushing time and technique. J Jpn Soc Periodontol. 1993, 35:674-680.
• [32]Suzuki T, Mochizuki S, Yamamoto S, Miyashita H, Yamamoto M: Plaque removal efficacy of a sonic toothbrush. J Jpn Soc Periodontol. 2007, 49:120-129.
• [33]Keukenmeester RS, Slot DE, Putt MS, Van der Weijden GA: The effect of medicated, sugar-free chewing gum on plaque and clinical parameters of gingival inflammation: a systematic review. Int J Dent Hyg. 2014, 12:2-16.
• [34]Ramberg P, Furuichi Y, Lindhe J, Gaffar A: A model for studying the effects of mouthrinses on de novo plaque formation. J Clin Periodontol 1992, 19:509-520.
• [35]Pizzo G, Guiglia R, La Cara M, Giuliana G, D’Angelo M: The effects of an amine fluoride/stannous fluoride and an antimicrobial host protein mouthrinse on supragingival plaque regrowth. J Periodontol 2004, 75:852-857.
• [36]Pizzo G, La Cara M, Licata ME, Pizzo I, D’Angelo M: The effects of an essential oil and an amine fluoride/stannous fluoride mouthrinse on supragingival plaque regrowth. J Periodontol 2008, 79:1177-1183.
• [37]Charles CH, Sharma NC, Galustians HJ, Qaqish J, McGuire JA, Vincent JW: Comparative efficacy of an antiseptic mouthrinse and an antiplaque/antigingivitis dentifrice. A six-month clinical trial. J Am Dent Assoc 2001, 132:670-675.