Antimicrobial and toxicological activities of five medicinal plant species from Cameroon Traditional Medicine

BMC Complementary and Alternative Medicine

Veronique B Penlap⁶  Bertrand Sandjon³  Abdel J Njouendou⁵  Emmanuel A Asongalem⁴  Peter F Nde¹  Anna L Njunda²  Dickson S Nsagha¹  Henri LF Kamga²  Jules CN Assob⁴ 

[1] Department of Public Health and Hygiene, Faculty of Health Sciences, University of Buea. P.O.Box 63, Buea, Cameroon;Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea. P.O.Box 63, Buea, Cameroon;Laboratoire Phytorica, P.O.Box 12521 Bonapriso, Douala. Cameroon;Medicine Programme, Department of Biomedical Sciences, Faculty of Health Sciences Laboratory, University of Buea. P.O.Box 63, Buea, Cameroon;Department of Biochemistry, Faculty of Science, University of Buea, P.O.Box 63, Buea, Cameroon;Laboratoire de Microbiologie Appliquée et de Pharmacologie Moléculaire, Département de Biochimie, Université de Yaoundé I. P.O. Box 812 Yaoundé, Cameroon

关键词: Cameroon Traditional Medicine;    kidney and liver function tests;    acute toxicity;    antimicrobial;    Piptadeniastum africana;    Phyllanthus muellerianus;   

Others  :  1232978
DOI  :  10.1186/1472-6882-11-70

 received in 2011-05-05, accepted in 2011-08-25,  发布年份 2011

【 摘 要 】

Background

Infectious diseases caused by multiresistant microbial strains are on the increase. Fighting these diseases with natural products may be more efficacious. The aim of this study was to investigate the in vitro antimicrobial activity of methanolic, ethylacetate (EtOAc) and hexanic fractions of five Cameroonian medicinal plants (Piptadeniastum africana, Cissus aralioides, Hileria latifolia, Phyllanthus muellerianus and Gladiolus gregasius) against 10 pathogenic microorganisms of the urogenital and gastrointestinal tracts.

Methods

The fractions were screened for their chemical composition and in vivo acute toxicity was carried out on the most active extracts in order to assess their inhibitory selectivity.

The agar well-diffusion and the micro dilution methods were used for the determination of the inhibition diameters (ID) and Minimum inhibitory concentrations (MIC) respectively on 8 bacterial species including two Gram positive species (Staphylococcus aureus, Enterococcus faecalis), and six Gram negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis,Shigella flexneri, Salmonella typhi) and two fungal isolates (Candida albicans, Candida krusei). The chemical composition was done according to Harbone (1976), the acute toxicity evaluation according to WHO protocol and the hepatic as well as serum parameters measured to assess liver and kidney functions.

Results

The chemical components of each plant's extract varied according to the solvent used, and they were found to contain alkaloids, flavonoids, polyphenols, triterpens, sterols, tannins, coumarins, glycosides, cardiac glycosides and reducing sugars. The methanolic and ethylacetate extracts of Phyllanthus muellerianus and Piptadeniastum africana presented the highest antimicrobial activities against all tested microorganisms with ID varying from 8 to 26 mm and MIC from 2.5 to 0.31 mg/ml. The in vivo acute toxicity study carried out on the methanolic extracts of Phyllanthus muellerianus and Piptadeniastrum africana indicated that these two plants were not toxic. At the dose of 4 g/kg body weight, kidney and liver function tests indicated that these two medicinal plants induced no adverse effect on these organs.

Conclusion

These results showed that, all these plant's extracts can be used as antimicrobial phytomedicines which can be therapeutically used against infections caused by multiresistant agents.

Phyllanthus muellerianus, Piptadeniastum africana, antimicrobial, acute toxicity, kidney and liver function tests, Cameroon Traditional Medicine

【 授权许可】

   
2011 Assob et al; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Iwu MM, Duncan AR, Okunji CO: New antimicrobials of Plant Origin. Perspectives on new crops and new uses. Edited by Janick J. ASHS Press, Alexandria, VA; 1999:457-462.
• [2]Pinner RS, Teutsch LS, Klug L, Graber J, Clarke M, Berkelman R: Trends in infectious diseases mortality in the United States. J Am Med Assoc 1996, 275:189-193.
• [3]Cos PM, Sindambiwe LW, Vlietinck AJ, Berghe DV: Bioassays for Antibacterial and Antifungal Activities. Edited by Mahabir P, Gupta S, Swami H, Karan V. Biological Screening of plant constituents. Training manual, International centre for science and high technology. Trieste; 2006:19-28.
• [4]Diallo D, Hveem B, Mahmoud MA, Betge G, Paulsen BS, Maiga A: An ethnobotanical survey of herbal drugs of Gourma district, Mali. Pharm Biol 1999, 37:80-91.
• [5]Kuete V: Potential of Cameroonian Plants and Derived Products against Microbial Infections: A Review. Planta Med 2010, 76:1479-1491.
• [6]Assob NJC, Weledji EP, Njunda AL, Bolimo F, Asongalem EA, Kamga FHL, Achidi EA, Penlap BV, Ndumbe PM: Bacteriological and mycological characterization of some pathogens of the urogenital tract in Buea subdivision (South West Region Cameroon). Health Sci & Dis 2009, 10:10-16.
• [7]Adjanohoun JE, Aboubabakar N, Dramane K, Ebot ME, Ekpere JA, Enow Orock EG, Focho D, Gbile ZO, Kamanyi A, Kamsu kom J, Keita A, Mbenkum T, Mbi CN, Mbiele AL, Mbome H, Mubiru NK, Nancy WL, Nkonmeneck B, Satabie B, Sofowara A, Tamze V, Wirmum VK: Contribution to ethnomedicinal and floristic studies in Cameroon. Traditional medicine and pharmacopoeia. Addis Ababa. CST/OUA; 1996.
• [8]Bruneton J: Pharmacognosie, phytochimie des plantes médicinales. 3rd edition. Tec-Et Doc. Edition médicale. Paris; 2000.
• [9]Harbone JB: Phytochemical methods. A guide to modern techniques of plant analyses. Chapman and hall, London; 1976:150.
• [10]Berghe VA, Vlietinck AJ: Screening Methods for Antibacterial and Antiviral Agents from Higher plants. In Methods of plants Biochemistry. Academic Press Limited. New York; 1991:47-69.
• [11]Perez C, Paul M, Bazerque P: An Antibiotic assay by the agar well-diffusion method. Acta Bio Med Exp 1990, 15:113-115.
• [12]Collins CH, Lynes PM, Grange JM: Microbiological Methods. 7th edition. Butterwort-Heinemann Ltd., Britain; 1995:175-190.
• [13]WHO: Research guidelines for evaluating the safety and efficacy of herbal medecine. Manila, Phillipines; 1992:38.
• [14]Berhens B, Karber G: "Mathematics for naturalists and agriculturalists". PWN, Warsaw; 1983:218-219.
• [15]Delongeas JL, Burnel D, Netter P, Grignon M, Mur JM, Royer R, Grignon G: Toxicité et pharmacocinétique de l'oxychlorure de zirconium chez la souris et chez le rat. J Pharmacol 1983, 14:437-447.
• [16]Shorderet M: Des concepts fondamentaux aux applications thérapeutiques. Pharmacologie. Edition Slatkine. Genève; 1989:30-43.
• [17]Lu FC: Données générales, Procédures d'évaluation, organes cibles et évaluation du risque. Toxicologie. Masson, Paris; 1992:3-87.
• [18]Reitman S, Frankel S: A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. American J Clinl Pathol 1957, 28-58.
• [19]Cheesbrough M: Medical Laboratory Manual for tropical countries. Vol. 2 Microbiology. EIBS pub. Low price Edition; 1985.
• [20]Wilbur KM, Berhein F, Shapiro OW: Dosage du malondialdehyde. Arch Biophys 1949, 24:305.
• [21]Ellman GL: Tissue sulphurhidryl groups. Arch Biophys 1949, 82:70-77.
• [22]Wonnacott TH, Wonnacott RJ: Statistique: Economie-Gestion-Sciences Médecine. 4th edition. Economia. Paris; 1995.
• [23]Houghton P, Hawkes RP, Jane E: Naphtoquinones and an alkaloid from roots of Newbouldia laevis. Phytochem 1994, 35:1602-3.
• [24]Tsuchiya HMS, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M, Tanaka T, Iinuma M: Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. J Ethnopharmacol 1996, 50:27-34.
• [25]Thornes RD: Clinical and biological observations associated with coumarins. In Coumarins: biology, applications and mode of action. Edited by O'Kennedy R, Thornes RD. John Wiley & Sons, Inc., New York, N.Y.; 1997:256.
• [26]Phillipson JD, O'Neill MJ: New leads to the treatment of protozoal infections based on natural product molecules. Acta Pharm Nord 1987, 1:131-144.
• [27]Mbouangouere RN, Tane P, Ngamga D, Djemgou P, Choudhary MI, Ngadjui BT: Piptaderol from Piptadenia africana. Afr J Trad CAM 2007, 4:294-298.
• [28]De Boer HJ, Kool A, Broberg A, Mziray WR, Hedberg I, Levenfors JJ: Antifungal and antibacterial activity of some herbal remedies from Tanzania. J Ethnopharmacol 2005, 96:461-469.
• [29]Schmelzer GH: Hilleria latifolia (Lam.) H. Walter. Volume 11. Edited by Schmelzer GH, Gurib-Fakim A. Prota. Medicinal plants/Plantes médicinales [CD-Rom]. PROTA, Wageningen, Pays Bas; 2007.
• [30]Buwa LV, Staden JV: Antibacterial and antifungal activity of traditional medicinal plants used against venereal diseases in South Africa. J Ethnopharmacol 2006, 103:139-142.
• [31]Parekh J, Jadeja S, Chanda : Efficacy of Aqueous and Methanol Extracts of some Medicinal Plants for Potential Antibacterial Activity. Turk J Biol 2005, 2:20-210.
• [32]Recio MC, Giner RM, Manez S, Gueho J, Julien HR, Hostettmann K: Investigations on the steroidal anti-inflammatory activity of triterpenoids from Diospyros leucome- las. Planta Med 1995, 61:9-12.
• [33]Supaluk P, Puttirat S, Rungrot C, Somsak R, Virapong P: New bioactive triterpenoids and antimalarial activity of Diospyros rubra lec. EXCLI J 2010, 9:1-10.
• [34]Vlietinck AJ, Van Hoof L, Totte J, Lasure A, Vanden Berghe D, Rwangobo PC, Mvukiyuniwami J: Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties. J Ethnopharmacol 1995, 46:31-47.
• [35]Abotsi WKM, Ainooson GK, Gyasi EB: Acute and Sub-acute Toxicity Studies of the Ethanolic Extract of the Aerial Parts of Hilleria Latifolia (Lam.) H. Walt. (Phytolaccaceae) in Rodents. West Afr J Pharma 2011, 22:27-35.
• [36]Assob Nguedia JC, F-X Etoa, Penlap Beng V, Lontsi D, Kuete V, Somo Moyou R: Anti-candidal property and acute toxicity of Gladiolus gregasius Baker (Iridaceae). Pharm Méd Trad Afr 2004, 13:149-159.
• [37]Ncube NS, Afolayan AJ, Okoh AI: Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Afr J Biotech 2008, 7:1797-1806.
• [38]Doughari JH, Sunday D: Antibacterial Activity of Phyllanthus muellerianus. Pharma Biol 2008, 46:400-405.
• [39]Brusotti G, Cesari I, Frassà G, Grisoli P, Dacarro C, Caccialanza G: Antimicrobial properties of stem bark extracts from Phyllanthus muellerianus (Kuntze) Excell. J Ethnopharmacol 2011, 03:042.
• [40]Gulati RK, Agarwal S, Agrawal SS: Hepatoprotective studies on Phyllanthus emblica Linn. and quercetin. Indian J Exp Biol 1995, 33:261-8.
• [41]Pornpen P, Patcharavadee S, Somlak P, Chaiyo C: The protective effects of Phyllanthus emblica Linn. extract on ethanol induced rat hepatic injury. J Ethnopharmacol 2006, 107:361-364.
• [42]Prakash A, Satyan KS, Wahi SP, Singh RP: Comparative hepatoprotective activity of three Phyllanthus species, P. urinaria, P. niruri and P. simplex, on carbon tetrachloride induced liver injury in the rat. Phytother Res 2006, 9:594-596.
• [43]Panchabhai TS, Ambarkhane SV, Joshi AS, Samant BD, Rege NN: Protective effect of Tinospora cordifolia, Phyllanthus emblica and their combination against antitubercular drugs induced hepatic damage: an experimental study. Phytotherapy research PTR (2008) Phytother Res 2008, 22:646-650.
• [44]Wanda HaschekM, Colin RousseauxG, Matthew WalligA: The Liver, in Fundamentals of Toxicologic Pathology (Second Edition). 2010, 197-235.
• [45]Dweck AC, Mitchell D: Emblica officinalis [Syn: Phyllanthus emblica] or Amla: the Ayurvedic wonder. Chesham Chemicals Ltd. London; 2002.
• [46]Jeong HG: Inhibition of cytochrome P450 2E1 expression by oleanolic acid: hepatoprotective effects against carbon tetrachloride-induced hepatic injury. Toxicol Lett 1999, 105:215-222.
• [47]Liu J, Liu Y, Klaassen CD: Protective effect of oleanolic acid against chemical-induced acute necrotic liver injury in mice. Acta Pharm Sin 1995, 16:97-102.
• [48]Balanehru S, Nagarajan B: Protective effect of oleanolic acid and ursolic acid against lipid peroxidation. Biochem Int 1991, 24:981-990.