A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max

BMC Research Notes

Melissa G Mitchum³  Thomas J Baum²  Steven A Whitham²  John Hill²  Parijat S Juvale²  Nathan W Gross¹  Greg Yeckel⁴  Robert Heinz³  Pramod K Kandoth³ 

[1]Current address: Beadle Center for Biotechnology, University of Nebraska, Lincoln NE, 68588, USA

[2]Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, USA

[3]Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA

[4]Current address: Pioneer Hi-Bred, Johnston IA, 50131, USA

关键词: VIGS;    Virus-induced gene silencing;    Resistance;    Cyst nematode;    Soybean;    SCN;    Resistance;    Heterodera glycines;   

Others  :  1142293
DOI  :  10.1186/1756-0500-6-255

 received in 2012-12-19, accepted in 2013-07-03,  发布年份 2013

【 摘 要 】

Background

Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

Findings

Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.

Conclusions

The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.

【 授权许可】

   
2013 Kandoth et al.; licensee BioMed Central Ltd.

【 图 表 】

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