Antioxidant activity of pomegranate juice reduces acute lung injury secondary to hyperoxia in an animal model

BMC Research Notes

Marwan El Sabban¹  Ghazi Zaatari³  Alain Rizkallah²  Yasmine Hashem²  Hala Bitar²  Aline Khayat²  Ahmad Husari² 

[1]Department of Biochemistry, American University of Beirut, Beirut, Lebanon

[2]Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, American University of Beirut, P.O. Box 11-236, Riad El Solh, Beirut 1107 2020, Lebanon

[3]Department of Pathology & Laboratory Medicine, American University of Beirut, Beirut, Lebanon

关键词: Inflammatory mediators;    Hyperoxia;    Acute lung injury;    Antioxidants;    Reactive oxygen species;   

Others  :  1129290
DOI  :  10.1186/1756-0500-7-664

 received in 2014-02-04, accepted in 2014-08-28,  发布年份 2014

【 摘 要 】

Background

Hyperoxia triggers the release of toxic reactive oxygen species (ROS). Pomegranate Juice (PJ) is a rich source of potent antioxidants. We assessed the effects of PJ supplementation on Acute Lung Injury (ALI) in adult rats exposed to hyperoxia for 5 days.

Methods

Adult rats were divided into four different groups: control, hyperoxia, hyperoxia + PJ and PJ. Animals were placed in chambers containing either room air or oxygen above 95% for a total of 5 days. Two different PJ concentrations were utilized and the control group received placebo water. Animals were euthanized and their lungs were excised. Assessment of lung injury was accomplished by: a) wet to dry ratio (W/D) method, b) measurement of albumin concentration in the bronchoalveolar lavage fluid (BALF), c) oxidative stress, d) histological evaluation of the lung e) apoptosis and f) transcriptional expression levels of the inflammatory mediators IL-1β, IL-6 and TNF-alpha.

Results

An increase in the W/D and albumin leak was noted in Hyperoxia (p < 0.05). Those findings were attenuated by the higher dose of PJ supplementation. Hyperoxia increased ROS production. Again PJ significantly reduced oxidative stress. Lung sections showed significant reduction in inflammation, edema, and infiltrating neutrophils in Hyperoxia + 80 μmol/kg when compared with Hyperoxia. TUNEL demonstrated significant apoptosis in the Hyperoxia, which was diminished in the Hyperoxia + 80 μmol/kg. Furthermore, increase in IL-1β and IL-6 was noted in Hyperoxia. Again, 80 μmol/kg of PJ significantly reduced the expression of inflammatory mediators.

Conclusion

In this animal model, PJ supplementation attenuated ALI associated with hyperoxia.

【 授权许可】

   
2014 Husari et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

【 参考文献 】

• [1]Clark JM, Lambertson CJ: Pulmonary oxygen toxicity. Pharmacol Rev 1971, 23:37-133.
• [2]Asikainen TM, White CW: Pulmonary antioxidant defenses in the preterm newborn with respiratory distress and bronchopulmonary dysplasia in evolution: implications for antioxidant therapy. Antioxid Redox Signal 2004, 1:155-167.
• [3]Asikainen TM, White CW: Pulmonary antioxidant defenses in the preterm newborn with respiratory distress and bronchopulmonary dysplasia in evolution: implications for antioxidant therapy. Antioxid Redox Signal 2004, 6:155-167.
• [4]Boveris A, Chance B: The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen. Biochem J 1973, 134:707-716.
• [5]Gutowski M, Kowalczyk S: A study of free radical chemistry: their role and pathophysiological significance. Acta Biochim Pol 2013, 60:1-16.
• [6]Husari AW, Dbaibo GS, Bitar H, Khayat A, Panjarian S, Nasser M, Bitar FF, El-Sabban M, Zaatari G, Mroueh SM: Apoptosis and the activity of ceramide, Bax and Bcl-2 in the lungs of neonatal rats exposed to limited and prolonged hyperoxia. Respir Res 2006, 7:100. BioMed Central Full Text
• [7]Davies KJ: Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life 2000, 50:279-289.
• [8]Saugstad OD: Bronchopulmonary dysplasia-oxidative stress and antioxidants. Semin Neonatol 2003, 8:39-49.
• [9]Perrone S, Tataranno ML, Buonocore G: Oxidative stress and bronchopulmonary dysplasia. J Clin Neonatol 2012, 1:109-114.
• [10]Chang LL, Subramaniam M, Yoder BA, Day BJ, Ellison MC, Sunday ME, Crapo JD: A catalytic antioxidant attenuates alveolar structural remodeling in bronchopulmonary dysplasia. Am J Respir Crit Care Med 2003, 167:57-64.
• [11]Perveen S, Patel H, Arif A, Younis S, Codipilly CN, Ahmed M: Role of EC-SOD overexpression in preserving pulmonary angiogenesis inhibited by oxidative stress. PLoS One 2012, 7(12):e51945. doi:10.1371/journal.pone.0051945. Epub 2012 Dec 20. PubMed PMID: 23284826; PubMed Central PMCID: PMC3527376
• [12]Jung O, Marklund SL, Geiger H, Pedrazzini T, Busse R, Brandes RP: Extracellular superoxide dismutase is a major determinant of nitric oxide bioavailability: in vivo and ex vivo evidence from ecSOD-deficient mice. Circ Res 2003, 93:622-629.
• [13]Berkelhamer SK, Farrow KN: Developmental regulation of antioxidant enzymes and their impact on neonatal lung disease. Antioxid Redox Signal 2014. [Epub ahead of print] PubMed PMID: 24295375
• [14]Tyson JE, Wright LL, Oh W, Kennedy KA, Mele L, Ehrenkranz RA, Stoll BJ, Lemon JA, Stevenson DK, Bauer CR, Korones SB, Fanaroff AA: Vitamin A supplementation for extremely-low-birth-weight infants. National Institute of Child Health and Human Development Neonatal Research Network. N Engl J Med 1999, 340:1962-1968.
• [15]Lee JW, Davis JM: Future applications of antioxidants in premature infants. Curr Opin Pediatr 2011, 23:161-166.
• [16]Faria A, Calhau C: The bioactivity of pomegranate: impact on health and disease. Crit Rev Food Sci Nutr 2011, 51:626-634.
• [17]Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA: Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem 2000, 48:4581-4589.
• [18]Seeram NP, Aviram M, Zhang Y, Henning SM, Feng L, Dreher M, Heber DJ: Comparison of antioxidant potency of commonly consumed polyphenol-rich beverages in the United States. Agric Food Chem 2008, 56:1415-1422.
• [19]Borges G, Crozier A: HPLC-PDA-MS fingerprinting to assess the authenticity of pomegranate beverages. Food Chem 2012, 135:1863-1867.
• [20]Cerdá B, Cerón JJ, Tomás-Barberán FA, Espín JC: Repeated oral administration of high doses of the pomegranate ellagitannin punicalagin to rats for 37 days is not toxic. J Agric Food Chem 2003, 51:3493-3501.
• [21]Nagato A, Silva FL, Silva AR, Bezerra FS, Oliveira ML, Belló-Klein A, Cristovao Porto L, Santos Valenca S: Hyperoxia-induced lung injury is dose dependent in Wistar rats. Exp Lung Res 2009, 35:713-728.
• [22]Ozdemir R, Yurttutan S, Talim B, Uysal B, Erdeve O, Oguz SS, Dilmen U: Colchicine protects against hyperoxic lung injury in neonatal rats. Neonatology 2012, 102:265-269.
• [23]Nagato AC, Bezerra FS, Lanzetti M, Lopes AA, Silva MA, Porto LC, Valença SS: Time course of inflammation, oxidative stress and tissue damage induced by hyperoxia in mouse lungs. Int J Exp Pathol 2012, 93:269-278.
• [24]Jain M, Chandel NS: Rethinking antioxidants in the intensive care unit. Am J Respir Crit Care Med 2013, 188:1283-1285.
• [25]Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, Heber D: In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem 2005, 16:360-367.