Thymoquinone attenuates cisplatin-induced hepatotoxicity via nuclear factor kappa- β

BMC Complementary and Alternative Medicine

Ahmed Amir Radwan Sayed¹  Abdulrahman L Al-Malki² 

[1]Chemistry Department, Faculty of Science, Minia University, El- Minia, Egypt

[2]Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O Box 80203, Jeddah, Saudi Arabia

关键词: IL-1β;    TNFα;    hepatotoxicity;    CP;    NF-κB;    Nigella Sativa;   

Others  :  1087091
DOI  :  10.1186/1472-6882-14-282

 received in 2014-05-07, accepted in 2014-07-25,  发布年份 2014

【 摘 要 】

Background

Cisplatin (CP) is known as a potent anti-cancer drug. The most therapeutic adverse effect of CP is induced hepatotoxicity. In the present study, the protective effect of thymoquinone (TQ) on CP-induced hepatotoxicity was studied.

Methods

Wistar rats were divided into three groups (15 rats each). Group 1 served as the control group. Group 2 rats were injected ip with a single dose of CP (12 mg/kg b.w, i.p.). Group 3 rats were orally pre-treated with TQ (500 mg. kg⁻¹. day⁻¹) for one month, then the animals were injected i.p with CP 12 mg.kg⁻¹.

Results

The beneficial effects of TQ with its antioxidant/anti-inflammatory effects were observed. Injection of rats with CP markedly affected the liver functions and histopathological changes. The antioxidant enzyme activities and reduced glutathione (GSH) contents were significantly decreased while the levels of malondialdehyde (MDA) significantly increased. The electromobility shift assay (EMSA) showed a significant activation of NF-κB-p65 in the rat liver injected with CP. Furthermore, the expression and concentrations of inflammatory tumor necrosis factor (TNF-α), nitric oxide synthetase (iNOS), and interleukin (IL-1β) were markedly elevated in the CP injected rats. The administration of TQ improved all the altered functions, histopathology of the liver and attenuated the activated NF-κB. The antioxidant enzyme activities (glutathione peroxidase and glutathione –S transferase) of the rat livers were markedly increased while MDA was reduced as a result of TQ administration. In addition, the expression of TNF-α, iNOS, and IL-1β were markedly reduced.

Conclusion

It was concluded that, TQ has potential benefits in the prevention of the onset and progression of CP induced hepatotoxicity.

【 授权许可】

   
2014 Al-Malki and Sayed; licensee BioMed Central Ltd.

【 图 表 】

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