Antinociceptive effect of ethanolic extract of Selaginella convoluta in mice

BMC Complementary and Alternative Medicine

JacksonRobertoGuedesdaSilva Almeida⁴  Alexsandro Branco²  Carla Rodrigues Cardoso Branco²  Patrícia Kauanna Fonseca Damasceno²  Lucindo José Quintans-Júnior³  Jullyana de Souza Siqueira³  André Paviotti Fontana¹  JoséAlvesdeSiqueira Filho¹  Julianeli Tolentino de Lima⁴  Xirley Pereira Nunes⁴  Pedro Guilherme S de Sá⁴ 

[1] Centro de Referência para Recuperação de Áreas Degradadas da Caatinga, Petrolina, Pernambuco, 56.300-000, Brazil;Laboratório de Fitoquímica, Departamento de Saúde, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia, 44.036-900, Brazil;Departamento de Fisiologia, Universidade Federal de Sergipe (DFS/UFS), Campus Universitário “Prof. Aloísio de Campos”, São Cristóvão, Sergipe, 49.100-000, Brazil;Núcleo de Estudos e Pesquisas de Plantas Medicinais, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco, 56.304-205, Brazil

关键词: Pain;    Analgesic;    Selaginellaceae;    Selaginella convoluta;   

Others  :  1231534
DOI  :  10.1186/1472-6882-12-187

 received in 2012-01-12, accepted in 2012-10-17,  发布年份 2012

【 摘 要 】

Background

Selaginella convoluta (Arn.) Spring (Selaginellaceae), commonly known as “jericó”, is a medicinal plant found in northeastern Brazil. S. convoluta is used in folk medicine as an antidepressant, aphrodisiac, diuretic, analgesic, anti-inflammatory and it is used to combat amenorrhea, coughing and bleeding. This study was performed to evaluate the antinociceptive effects of ethanolic extract from S. convoluta in mice exposed to chemical and thermal models of nociception.

Methods

Preliminary phytochemical analysis of the ethanolic extract was performed. The ethanolic extract from Selaginella convoluta (Sc-EtOH) was examined for its intraperitoneal (i.p.) antinociceptive activity at the doses of 100, 200 and 400 mg/kg body weight. Acetic acid-induced writhing, formalin injection and hot plate tests were used to evaluate the antinociceptive activity of Sc-EtOH extract. The rota-rod test was used to evaluate motor coordination.

Results

A preliminary analysis of Sc-EtOH revealed that it contained phenols, steroids, terpenoids and flavonoids. In the acetic acid-induced writhing test, mice treated with Sc-EtOH (100, 200 and 400 mg/kg, i.p.) exhibited reduced writhing (58.46, 75.63 and 82.23%, respectively). Secondly, Sc-EtOH treatment (100, 200 and 400 mg/kg, i.p.) decreased the paw licking time in mice during the first phase of the formalin test (by 44.90, 33.33 and 34.16%, respectively), as well as during the second phase of the test (by 86.44, 56.20 and 94.95%, respectively). Additionally, Sc-EtOH treatment at doses of 200 and 400 mg/kg increased the latency time in the hot plate test after 60 and 90 minutes, respectively. In addition, Sc-EtOH did not impair motor coordination.

Conclusion

Overall, these results indicate that Sc-EtOH is effective as an analgesic agent in various pain models. The activity of Sc-EtOH is most likely mediated via the inhibition of peripheral mediators and central inhibitory mechanisms. This study supports previous claims of traditional uses for S. convoluta.

【 授权许可】

   
2012 de Sá et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 1.

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