MicroRNA-34a inhibits human trophoblast cell invasion by targeting MYC

BMC Cell Biology

Tao Meng¹  Chunxiao Tong¹  Jing Liu¹  Haiying Chen¹  Manni Sun¹ 

[1] Department of Obstetrics, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang 110001, People’s Republic of China

关键词: Preeclampsia;    Trophoblast invasion;    MYC;    microRNA-34a;   

Others  :  1224005
DOI  :  10.1186/s12860-015-0068-2

 received in 2015-05-03, accepted in 2015-08-21,  发布年份 2015

【 摘 要 】

Background

Preeclampsia, one of the major disorders of pregnancy, is characterized by inadequate trophoblast invasion and defective trophoblast-mediated remodeling of placental vasculature. MicroRNA-34a (miR-34a) has been found to be aberrantly expressed in the placentas of preeclamptic patients, yet its role in placental development and in the pathogenesis of preeclampsia remains elusive.

Results

The levels of miR-34a in the placentas of 20 preeclamptic patients and 20 healthy subjects were determined by real time-PCR, and miR-34a was found significantly elevated in the preeclamptic placentas. Further, the function of miR-34a in trophoblast cells was investigated by overexpressing miR-34a in JEG-3 trophoblast cell line. Overexpression of miR-34a in JEG-3 cells inhibited cell proliferation, migration and invasion. In addition, elevated expression of miR-34a reduced the expression of both endogenous and ectopic MYC. Moreover, we identified that MYC mRNA was a direct target of miR-34a in JEG-3 cells by dual luciferase reporter assay, and found that downregulation of MYC expression by miR-34a targeting significantly reduced the invasiveness of JEG-3 cells.

Conclusions

Our findings provide preliminary evidence for the diverse functions of miR-34a in trophoblast biology, and suggest that miR-34a suppresses trophoblast invasion by directly targeting MYC.

【 授权许可】

   
2015 Sun et al.

【 图 表 】

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