Diversity of endophytic fungi and screening of fungal paclitaxel producer from Anglojap yew, Taxus x media

BMC Microbiology

Yong Wang²  Na Zhao²  Ying-Ying Yang¹  Zhi-Qiang Xiong² 

[1]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

[2]Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

关键词: Taxol gene cluster;    Guignardia mangiferae;    Endophytic fungi;    Paclitaxel;    Taxus x media;   

Others  :  1221687
DOI  :  10.1186/1471-2180-13-71

 received in 2012-11-12, accepted in 2013-01-31,  发布年份 2013

【 摘 要 】

Background

Endophytic fungi represent underexplored resource of novel lead compounds and have a capacity to produce diverse class of plant secondary metabolites. Here we investigated endophytic fungi diversity and screening of paclitaxel-producing fungi from Taxus x media.

Results

Eighty-one endophytic fungi isolated from T. media were grouped into 8 genera based on the morphological and molecular identification. Guignardia and Colletotrichum were the dominant genera, whereas the remaining genera were infrequent groups. The genera Glomerella and Gibberella were first reported in Taxus. Three representative species of the distinct genera gave positive hits by molecular marker screening and were capable of producing taxol which were validated by HPLC-MS. Among these 3 taxol-producing fungi, the highest yield of taxol was 720 ng/l by Guignardia mangiferae HAA11 compared with those of Fusarium proliferatum HBA29 (240 ng/l) and Colletotrichum gloeosporioides TA67 (120 ng/l). This is the first report of taxol producer from Guignardia. Moreover, the lower similarities of ts and bapt between microbial and plant origin suggested that fungal taxol biosynthetic cluster might be repeatedly invented during evolution, nor horizontal gene transfer from Taxus species.

Conclusions

Taxol-producing endophytic fungi could be a fascinating reservoir to generate taxol-related drug lead and to elucidate the remained 5 unknown genes or the potential regulation mechanism in the taxol biosynthesis pathway.

【 授权许可】

   
2013 Xiong et al.; licensee BioMed Central Ltd.

【 图 表 】

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