Broad application of a simple and affordable protocol for isolating plant RNA

BMC Research Notes

Rosa Lozano-Durán²  Cyril Zipfel³  Ana Arruabarrena¹  Lena Stransfeld³  Daniel Couto³ 

[1] National Agricultural Research Institute (INIA), Estación Experimental INIA Salto Grande, Salto CP50000, Uruguay;Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, Shanghai 201602, China;The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom

关键词: Protocol;    Affordable;    Wheat;    Tomato;    Arabidopsis;    RNA;   

Others  :  1177886
DOI  :  10.1186/s13104-015-1119-7

 received in 2014-06-24, accepted in 2015-04-01,  发布年份 2015

【 摘 要 】

Background

Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner.

Findings

We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents.

Conclusions

Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.

【 授权许可】

   
2015 Couto et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

【 参考文献 】

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• [3]Weigel D, Glazebrook J: Arabidopsis: a laboratory manual. Cold Spring Harbor Laboratory Press, New York: Cold Spring Harbor; 2002.
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