Differential efficiency in exogenous DNA acquisition among closely related Salmonella strains: implications in bacterial speciation

BMC Microbiology

Shu-Lin Liu²  Ye Feng¹  Gui-Rong Liu⁴  Randal N Johnston³  Da-Ling Zhu⁵  Ang Li⁶  Yong-Guo Li⁷  Xia Deng⁴  Yang Li⁴  Xu-Yao Wang⁴  Lu Yu⁴  Le Tang²  Hong-Xia Bao² 

[1] Institute for Translational Medicine, Zhejiang University School of Medicine, Hangzhou, China;Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada;Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Canada;HMU-UCFM Centre for Infection and Genomics, Harbin Medical University, Harbin, China;College of Pharmacy of Daqing Campus, Harbin Medical University, Harbin, China;Department of Biostatistics, Harbin Medical University, Harbin, China;Department of Infectious Diseases of First Affiliated Hospital, Harbin Medical University, Harbin, China

关键词: Transformation;    Transduction;    Salmonella;    Homologous recombination;    Bacterial speciation;   

Others  :  1140939
DOI  :  10.1186/1471-2180-14-157

 received in 2013-07-18, accepted in 2014-06-10,  发布年份 2014

【 摘 要 】

Background

Acquisition of exogenous genetic material is a key event in bacterial speciation. It seems reasonable to assume that recombination of the incoming DNA into genome would be more efficient with higher levels of relatedness between the DNA donor and recipient. If so, bacterial speciation would be a smooth process, leading to a continuous spectrum of genomic divergence of bacteria, which, however, is not the case as shown by recent findings. The goal of this study was todetermine if DNA transfer efficiency is correlated with the levels of sequence identity.

Results

To compare the relative efficiency of exogenous DNA acquisition among closely related bacteria, we carried out phage-mediated transduction and plasmid-mediated transformation in representative Salmonella strains with different levels of relatedness. We found that the efficiency was remarkably variable even among genetically almost identical bacteria. Although there was a general tendency that more closely related DNA donor-recipient pairs had higher transduction efficiency, transformation efficiency exhibited over a thousand times difference among the closely related Salmonella strains.

Conclusion

DNA acquisition efficiency is greatly variable among bacteria that have as high as over 99% identical genetic background, suggesting that bacterial speciation involves highly complex processes affected not only by whether beneficial exogenous DNA may exist in the environment but also the “readiness” of the bacteria to accept it.

【 授权许可】

   
2014 Bao et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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