Effect of environmental stress factors on the uptake and survival of Campylobacter jejuni in Acanthamoeba castellanii

BMC Microbiology

Carole Creuzenet⁴  Dang Duong Bang³  Anders Wolff¹  Klaus Qvortrup²  Xuan Thanh Bui⁴ 

[1] BioLabChip group, Department of Micro and Nanotechnology, Technical University of Denmark, Kgs Lyngby, DK-2800, Denmark;Department of Biomedical Sciences, University of Copenhagen, Copenhagen N, 2200, Denmark;Laboratory of Applied Micro and Nanotechnology, National Food Institute, Technical University of Denmark, Søborg, DK-2860, Denmark;Department of Microbiology and Immunology, Infectious Diseases Research Group, University of Western Ontario, London, ON N6A 5C1, Canada

关键词: Virulence;    Environmental stresses;    Acanthamoeba castellanii;    Campylobacter jejuni;   

Others  :  1221710
DOI  :  10.1186/1471-2180-12-232

 received in 2012-05-03, accepted in 2012-10-03,  发布年份 2012

【 摘 要 】

Background

Campylobacter jejuni is a major cause of bacterial food-borne illness in Europe and North America. The mechanisms allowing survival in the environment and transmission to new hosts are not well understood. Environmental free-living protozoa may facilitate both processes. Pre-exposure to heat, starvation, oxidative or osmotic stresses encountered in the environment may affect the subsequent interaction of C. jejuni with free-living protozoa. To test this hypothesis, we examined the impact of environmental stress on expression of virulence-associated genes (ciaB, dnaJ, and htrA) of C. jejuni and on its uptake by and intracellular survival within Acanthamoeba castellanii.

Results

Heat, starvation and osmotic stress reduced the survival of C. jejuni significantly, whereas oxidative stress had no effect. Quantitative RT-PCR experiments showed that the transcription of virulence genes was slightly up-regulated under heat and oxidative stresses but down-regulated under starvation and osmotic stresses, the htrA gene showing the largest down-regulation in response to osmotic stress. Pre-exposure of bacteria to low nutrient or osmotic stress reduced bacterial uptake by amoeba, but no effect of heat or oxidative stress was observed. Finally, C. jejuni rapidly lost viability within amoeba cells and pre-exposure to oxidative stress had no significant effect on intracellular survival. However, the numbers of intracellular bacteria recovered 5 h post-gentamicin treatment were lower with starved, heat treated or osmotically stressed bacteria than with control bacteria. Also, while ~1.5 × 10³ colony forming unit/ml internalized bacteria could typically be recovered 24 h post-gentamicin treatment with control bacteria, no starved, heat treated or osmotically stressed bacteria could be recovered at this time point. Overall, pre-exposure of C. jejuni to environmental stresses did not promote intracellular survival in A. castellanii.

Conclusions

Together, these findings suggest that the stress response in C. jejuni and its interaction with A. castellanii are complex and multifactorial, but that pre-exposure to various stresses does not prime C. jejunifor survival within A. castellanii.

【 授权许可】

   
2012 Bui et al.; licensee BioMed Central Ltd.

【 图 表 】

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