Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment

BMC Complementary and Alternative Medicine

Hsiao-Chuan Wen³  Su-Chiu Chen⁴  Hu-Yi Tseng¹  Ming Shuang Hsieh²  Fei Shish Yang⁴  Chi-Long Juang² 

[1] Taipei City Hospital, Yangming Branch, Department of Radiological Technology, Taipei, Taiwan;Department of Medical Imaging and Radiological Technology, Yuanpei University, Hsinchu 30015, Taiwan;Department of Optometry, Yuanpei University, Hsinchu 30015, Taiwan;Mackay Memorial Hospital, Department of Radiological Technology, Taipei, Taiwan

关键词: LDH;    DPPH;    Tithonia diversifolia ethanolic extracts;    Apparent diffusion coefficient;    Spinal cord injury;   

Others  :  1085415
DOI  :  10.1186/1472-6882-14-447

 received in 2013-07-04, accepted in 2014-08-18,  发布年份 2014

【 摘 要 】

Background

Spinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality. Treatments to improve the neurological outcome of secondary injury are considered as important issues. The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.

Methods

The anti-oxidation properties were tested in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Rat liver cells (clone-9) were treated with various doses of TDE (0 ~ 50 μg/ml) before exposed to 250 μM H₂O₂ and cell survival was determined by MTT and LDH assays. We performed water apparent diffusion coefficient (ADC) map in MR techniques to investigate the efficacy of TDE treatment on SCI animal model. We performed T5 laminectomy and compression (50 g, 1 min) to induce SCI. PHILIP 3.0 T MRI was used to image 24 male Sprague–Dawley rats weighing 280–320 g. Rats were randomly divided into three groups: sham group, SCI group, SCI treated with TDE group. The MRI images were taken and ADC were acquired before and after of treatment of TDE (50 mg/kg B. W. orally, 5 days) in SCI model.

Results

TDE protected clone-9 cells against H₂O₂-induced toxicity through DPPH scavenging mechanism. In addition, SCI induced the increase in ADC after 6 hours. TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.

Conclusion

Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

【 授权许可】

   
2014 Juang et al.; licensee BioMed Central Ltd.

【 图 表 】

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