Infection of human monocytes by Chlamydia pneumoniae and Chlamydia trachomatis: an in vitro comparative study

BMC Research Notes

Roberto Cevenini¹  Claudio Foschi¹  Paola Nardini¹  Manuela Donati¹  Claudia Cavallini²  Romana Fato³  Christian Bergamini³  Antonella Marangoni¹ 

[1]Microbiology, DIMES, University of Bologna, S.Orsola Hospital, via Massarenti 9, 40138 Bologna, Italy

[2]Laboratory of Molecular Biology and Stem Cell Engineering, DIMES, University of Bologna, Bologna, Italy

[3]FaBiT Department, University of Bologna, Bologna, Italy

关键词: Cytokines;    Reactive nitrogen species;    Reactive oxygen species;    Chlamydia trachomatis;    Chlamydia pneumoniae;    Human monocytes;   

Others  :  1133923
DOI  :  10.1186/1756-0500-7-230

 received in 2013-10-24, accepted in 2014-04-03,  发布年份 2014

【 摘 要 】

Background

An increasing number of studies suggest that chlamydiae can infect immune cells. The altered immune cell function could contribute to the progression of several chronic inflammatory diseases.

The aim of this study was to comparatively evaluate Chlamydia pneumoniae (CP) and Chlamydia trachomatis (CT) interactions with in vitro infected human blood monocytes.

Results

Fresh isolated monocytes were infected with viable CP and CT elementary bodies and infectivity was evaluated by recultivating disrupted monocytes in permissive epithelial cells.

The production of reactive oxygen and nitrogen species was studied in the presence of specific fluorescent probes. Moreover, TNF-α, INF-α, INF-β and INF-γ gene expression was determined.

CT clearance from monocytes was complete at any time points after infection, while CP was able to survive up to 48 hours after infection. When NADPH oxydase or nitric oxide synthase inhibitors were used, CT infectivity in monocytes was restored, even if at low level, and CT recovery’s rate was comparable to CP one.

CT-infected monocytes produced significantly higher levels of reactive species compared with CP-infected monocytes, at very early time points after infection. In the same meanwhile, TNF-α and INF-γ gene expression was significantly increased in CT-infected monocytes.

Conclusions

Our data confirm that CP, but not CT, is able to survive in infected monocytes up to 48 hours post-infection. The delay in reactive species and cytokines production by CP-infected monocytes seems to be crucial for CP survival.

【 授权许可】

   
2014 Marangoni et al.; licensee BioMed Central Ltd.

【 图 表 】

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