Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract

BMC Complementary and Alternative Medicine

Nur Azilah Abdullah³  Badrul Hisyam Zainudin³  Siti Salwa Abd Gani⁴  Puziah Hashim⁴  Amin Ismail²  Azrina Azlan¹  Azila Abdul Karim³ 

[1] Research Centre of Excellence, Nutrition and Non-communicable Disease, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;Cocoa Innovation and Technology Centre, Malaysian Cocoa Board, PT12621, Nilai Industrial Area, 71800 Nilai, Negeri Sembilan, Malaysia;Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

关键词: Cosmetic ingredient;    Sunscreen agent;    Skin whitening;    Anti-wrinkles;    Antioxidant;    Cocoa pod extract;   

Others  :  1086312
DOI  :  10.1186/1472-6882-14-381

 received in 2014-02-17, accepted in 2014-10-01,  发布年份 2014

【 摘 要 】

Background

Cocoa pod is an outer part of cocoa fruits being discarded during cocoa bean processing. Authors found out that data on its usage in literature as cosmetic materials was not recorded in vast. In this study, cocoa pod extract was investigated for its potential as a cosmetic ingredient.

Methods

Cocoa pod extract (CPE) composition was accomplished using UHPLC. The antioxidant capacity were measured using scavenging assay of 1,2-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching assay (BCB) and ferric reducing antioxidant power (FRAP). Inhibiting effect on skin degradation enzymes was carried out using elastase and collagenase assays. The skin whitening effect of CPE was determined based on mushroom tyrosinase assay and sun screening effect (UV-absorbance at 200-400 nm wavelength).

Results

LC-MS/MS data showed the presence of carboxylic acid, phenolic acid, fatty acid, flavonoids (flavonol and flavones), stilbenoids and terpenoids in CPE. Results for antioxidant activity exhibited that CPE possessed good antioxidant activity, based on the mechanism of the assays compared with ascorbic acid (AA) and standardized pine bark extract (PBE); DPPH: AA > CPE > PBE; FRAP: PBE > CPE > AA; and BCB: BHT > CPE > PBE. Cocoa pod extract showed better action against elastase and collagenase enzymes in comparison with PBE and AA. Higher inhibition towards tyrosinase enzyme was exhibited by CPE than kojic acid and AA, although lower than PBE. CPE induced proliferation when tested on human fibroblast cell at low concentration. CPE also exhibited a potential as UVB sunscreen despite its low performance as a UVA sunscreen agent.

Conclusions

Therefore, the CPE has high potential as a cosmetic ingredient due to its anti-wrinkle, skin whitening, and sunscreen effects.

【 授权许可】

   
2014 Abdul Karim et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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