Integrated proteomic and transcriptomic analysis of the Aedes aegypti eggshell

BMC Developmental Biology

Anthony A James²  Paul D Gershon³  Zhijian Tu¹  Marika F Walter⁴  Xiaofang Jiang¹  Pedro N Marinotti³  Rebeca Carballar-Lejarazú³  Jennifer Juhn³  Brian Nguyen³  Shao-Pei Chou⁵  Bianca B Kojin³  Tuan Ngo³  Osvaldo Marinotti³ 

[1] Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA;Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697, USA;Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA;Department of Biological Chemistry, School of Medicine, University of California, Irvine, CA 92697, USA;Department of Molecular Biology and Genetics, Cornell University, Cornell, NY 14850, USA

关键词: Mosquito;    Oogenesis;    Estivation;    Vitelline membrane;    Chorion;    Eggshell;    Aedes aegypti;   

Others  :  1084996
DOI  :  10.1186/1471-213X-14-15

 received in 2013-12-09, accepted in 2014-03-31,  发布年份 2014

【 摘 要 】

Background

Mosquito eggshells show remarkable diversity in physical properties and structure consistent with adaptations to the wide variety of environments exploited by these insects. We applied proteomic, transcriptomic, and hybridization in situ techniques to identify gene products and pathways that participate in the assembly of the Aedes aegypti eggshell. Aedes aegypti population density is low during cold and dry seasons and increases immediately after rainfall. The survival of embryos through unfavorable periods is a key factor in the persistence of their populations. The work described here supports integrated vector control approaches that target eggshell formation and result in Ae. aegypti drought-intolerant phenotypes for public health initiatives directed to reduce mosquito-borne diseases.

Results

A total of 130 proteins were identified from the combined mass spectrometric analyses of eggshell preparations.

Conclusions

Classification of proteins according to their known and putative functions revealed the complexity of the eggshell structure. Three novel Ae. aegypti vitelline membrane proteins were discovered. Odorant-binding and cysteine-rich proteins that may be structural components of the eggshell were identified. Enzymes with peroxidase, laccase and phenoloxidase activities also were identified, and their likely involvements in cross-linking reactions that stabilize the eggshell structure are discussed.

【 授权许可】

   
2014 Marinotti et al.; licensee BioMed Central Ltd.

【 图 表 】

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