Selected metabolites profiling of Orthosiphon stamineus Benth leaves extracts combined with chemometrics analysis and correlation with biological activities

BMC Complementary and Alternative Medicine

Zhari Ismail¹  Amin Malik Shah Abdul Majid²  Khamsah Suryati Mohd⁴  Mohammad Razak Hamdan³  Maha Mansour Abdelbari¹  Abdul Hakeem Memon¹  Mohd Shahrul Ridzuan Hamil¹  Noor Hafizoh Saidan¹ 

[1] Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia;Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia;Centre of Drug Research, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia;School of Agriculture and Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Tembila Campus, Besut, 22200, Terengganu, Malaysia

关键词: HCA;    PCA;    Chemometrics tools;    FTIR;    HPLC;    Lamiacea;    Orthosiphon stamineus;    Metabolites;   

Others  :  1233327
DOI  :  10.1186/s12906-015-0884-0

 received in 2015-05-12, accepted in 2015-09-28,  发布年份 2015

【 摘 要 】

Background

Studies on selected metabolites profiling of Orthosiphon stamineus extracts using chromatographic and spectroscopic techniques combined with chemometric tools have not been fully elucidated. Thus present study was performed to profile selected metabolites in O. stamineus leaves extracts using HPLC and FTIR combined with chemometric tools and correlated with biological activities.

Methods

Five different extracts were prepared using three methods; maceration, soxhlet and reflux. The extracts were analyzed using UV-Vis, HPLC and FTIR techniques. Analysis of selected primary and secondary metabolites was also evaluated. The antioxidant and cytotoxic activities of the extracts were evaluated. Chemometric tools were employed to classify the extracts based on HPLC analysis and FTIR fingerprints.

Results

The ethanolic extract using maceration characterized high content of phenolics and flavonoids, (rosmarinic acid and eupatorin) with high antioxidant activity. Ethanolic (50 %) and methanolic extracts using soxhlet showed high proteins and glycosaponins. Water extracts using reflux and maceration showed high polysaccharides. Methanolic extract (50 %) using soxhlet and methanolic extract using maceration showed strong cytotoxic effect against MCF7 and HCT116 cell lines, respectively. Antioxidant and cytotoxic activities showed significant correlation with selected primary and secondary metabolites. HPLC fingerprints combined with chemometrics showed the extracts have been clustered based on selected major peaks profile. FTIR fingerprints combined with chemometrics showed that the extracts have been clustered based on protein and polysaccharide contents.

Conclusion

Ten different extracts of O. stamineus have showed significant differences in the content of selected primary and secondary metabolites as well as the biological activities. Chemometric tools were able to classify and discriminate the distinctive features of extracts thus can be correlated with the biological activities.

【 授权许可】

   
2015 Saidan et al.

【 图 表 】

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