Glycation and oxidative stress in the failure of dental implants: a case series

BMC Research Notes

Annalisa Monaco¹  Roberto Gatto¹  Irma Ciarrocchi¹  Marco Severino¹  Eleonora Ortu¹  Davide Pietropaoli¹ 

[1] University of L’Aquila - Department of Life, Health and Environmental Sciences San Salvatore Hospital, Building Delta 6 – Unit of Dentistry - Via Vetoio, L’Aquila, 67100, Italy

关键词: ROS;    AGEs;    Advanced glycation end products;    Glycation;    Oxidative stress;    Periimplantitis;    Dental implants;   

Others  :  1142054
DOI  :  10.1186/1756-0500-6-296

 received in 2013-03-27, accepted in 2013-07-24,  发布年份 2013

【 摘 要 】

Background

The aim of this case series/control study is to investigate the presence of the Advanced Glycation End products (AGEs) and oxidative stress in periimplantitis.

The study group was composed of five dental implants, failed within 6 months after implantation, taken from 5 subjects (3 M/2 F) aged between 43–57 years and stored in isotonic liquid before freezing at -80°C, according to literature. All the implants had been placed using traditional submerged technique. The whole saliva was also collected using Salimetrics device and stored at -80°C, to assess molecular analysis. Two age-matched control groups were examined: they consisted of 5 subjects encountering dental extraction for chronic periodontal disease (2 M/3 F) and 5 healthy subjects (3 M/2 F) who needed extraction for dental trauma. Their whole saliva was collected with the same method. The implants and the tooth of control groups were processed to assess Western Blotting for identification of AGEs. The case/control whole saliva was used to perform ThioBarbituric Acid Reactive Substances (TBARS) for oxidative stress evaluation.

Findings

The Western Blotting analysis on periimplantitis and periodontal disease tissues showed marked increase of AGEs when compared to healthy control tissues. Also TBARS assay of whole saliva confirmed the expectations, showing higher oxidative stress levels in periimplantitis and periodontitis groups than in healthy group.

Conclusions

With the limitation of the sample size, these results showed that oxidative stress could be involved in the aetiology of periimplantitis. This hypothesis could lead to new therapeutic strategies in periimplantitis, using antioxidant approach in addition to conventional treatments.

【 授权许可】

   
2013 Pietropaoli et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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