Protein interactions of the transcription factor Hoxa1

BMC Developmental Biology

René Rezsohazy⁴  Marc Vidal²  Jean-Claude Twizere¹  Nicolas Simonis³  Isabelle Bergiers⁴  Sophie Remacle⁴  Julie Vandeputte⁴  Barbara Lambert⁴ 

[1] GIGA-R and Gembloux Agro Bio-Tech, Université de Liège, Liège, 4000, Belgium;Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA;Bioinformatique des Génomes et des Réseaux (BiGRe), Université libre de Bruxelles, Bruxelles, Belgium;Molecular and Cellular Animal Embryology group, Life Sciences Institute (ISV), Université Catholique de Louvain, Louvain-la-Neuve, 1348, Belgium

关键词: Interactome;    ORFeome;    Hoxa1;    Hox;   

Others  :  1086444
DOI  :  10.1186/1471-213X-12-29

 received in 2012-10-16, accepted in 2012-10-16,  发布年份 2012

【 摘 要 】

Background

Hox proteins are transcription factors involved in crucial processes during animal development. Their mode of action remains scantily documented. While other families of transcription factors, like Smad or Stat, are known cell signaling transducers, such a function has never been squarely addressed for Hox proteins.

Results

To investigate the mode of action of mammalian Hoxa1, we characterized its interactome by a systematic yeast two-hybrid screening against ~12,200 ORF-derived polypeptides. Fifty nine interactors were identified of which 45 could be confirmed by affinity co-purification in animal cell lines. Many Hoxa1 interactors are proteins involved in cell-signaling transduction, cell adhesion and vesicular trafficking. Forty-one interactions were detectable in live cells by Bimolecular Fluorescence Complementation which revealed distinctive intracellular patterns for these interactions consistent with the selective recruitment of Hoxa1 by subgroups of partner proteins at vesicular, cytoplasmic or nuclear compartments.

Conclusions

The characterization of the Hoxa1 interactome presented here suggests unexplored roles for Hox proteins in cell-to-cell communication and cell physiology.

【 授权许可】

   
2012 Lambert et al.; licensee BioMed Central Ltd.

【 图 表 】

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