Protective effects of flavonol isoquercitrin, against 6-hydroxy dopamine (6-OHDA) - induced toxicity in PC12 cells

BMC Research Notes

Nagaraja Haleagrahara¹  Ammu Radhakrishnan²  Kasthuri Bai Magalingam² 

[1] Discipline of Physiology and Pharmacology, School of Veterinary and Biomedical Sciences, Faculty of Medicine, Health and Molecular Sciences, James Cook University, Townsville 4811, Australia;Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia

关键词: Antioxidant Flavonoids 6-hydroxydopamine Parkinson’s disease Oxidative stress;   

Others  :  1134767
DOI  :  10.1186/1756-0500-7-49

 received in 2013-08-17, accepted in 2014-01-16,  发布年份 2014

【 摘 要 】

Background

Free radicals-induced neurodegeneration is one of the many causes of Parkinson’s disease (PD). This study investigated the neuroprotective effects of flavonol isoquercitrin against toxicity induced by 6-hydroxy-dopamine (6-OHDA) in rat pheochromocytoma (PC12) cells.

Methods

PC12 cells were pretreated with different concentrations of isoquercitrin for 4, 8 and 12 hours and incubated with 6-OHDA for 24 hours to induce oxidative cell damage.

Results

A significant cytoprotective activity was observed in isoquercitrin pre-treated cells in a dose-dependent manner. There was a significant increase (P < 0.01) in the antioxidant enzymes namely superoxide dismutase, catalase, glutathione peroxidase, and glutathione in isoquercitrin pretreated cells compared to cells incubated with 6-OHDA alone. Isoquercitrin significantly reduced (P < 0.01) lipid peroxidation in 6-OHDA treated cells. These results suggested that isoquercitrin protects PC 12 cells against 6-OHDA–induced oxidative stress.

Conclusions

The present study suggests the protective role of isoquercitrin on 6-hydroxydopamine-induced toxicity by virtue of its antioxidant potential. Isoquercitrin could be a potential therapeutic agent against neurodegeneration in Parkinson’s disease.

【 授权许可】

   
2014 Magalingam et al.; licensee BioMed Central Ltd.

【 图 表 】

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