Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming

BMC Genomics

Magnus Lucassen³  Hans-Otto Pörtner³  Christoph Held¹  Daniela Storch³  Felix Christopher Mark³  Melanie Schiffer³  Stephan Frickenhaus²  Lars Harms³ 

[1] Functional Ecology, Alfred Wegener Institute, Bremerhaven, Germany;Scientific Computing, Alfred Wegener Institute, Bremerhaven, Germany;Integrative Ecophysiology, Alfred Wegener Institute, Bremerhaven, Germany

关键词: Crustaceans;    Gene expression;    Warming;    Ocean acidification;    RNA-Seq;    Hyas araneus;   

Others  :  1140551
DOI  :  10.1186/1471-2164-15-789

 received in 2014-01-13, accepted in 2014-09-09,  发布年份 2014

【 摘 要 】

Background

Hypercapnia and elevated temperatures resulting from climate change may have adverse consequences for many marine organisms. While diverse physiological and ecological effects have been identified, changes in those molecular mechanisms, which shape the physiological phenotype of a species and limit its capacity to compensate, remain poorly understood. Here, we use global gene expression profiling through RNA-Sequencing to study the transcriptional responses to ocean acidification and warming in gills of the boreal spider crab Hyas araneus exposed medium-term (10 weeks) to intermediate (1,120 μatm) and high (1,960 μatm) PCO₂ at different temperatures (5°C and 10°C).

Results

The analyses reveal shifts in steady state gene expression from control to intermediate and from intermediate to high CO₂ exposures. At 5°C acid–base, energy metabolism and stress response related genes were upregulated at intermediate PCO₂, whereas high PCO₂ induced a relative reduction in expression to levels closer to controls. A similar pattern was found at elevated temperature (10°C). There was a strong coordination between acid–base, metabolic and stress-related processes. Hemolymph parameters at intermediate PCO₂ indicate enhanced capacity in acid–base compensation potentially supported by upregulation of a V-ATPase. The likely enhanced energy demand might be met by the upregulation of the electron transport system (ETS), but may lead to increased oxidative stress reflected in upregulated antioxidant defense transcripts. These mechanisms were attenuated by high PCO₂, possibly as a result of limited acid–base compensation and metabolic down-regulation.

Conclusion

Our findings indicate a PCO₂ dependent threshold beyond which compensation by acclimation fails progressively. They also indicate a limited ability of this stenoecious crustacean to compensate for the effects of ocean acidification with and without concomitant warming.

【 授权许可】

   
2014 Harms et al.; licensee BioMed Central Ltd.

【 图 表 】

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