Emilia coccinae (SIMS) G Extract improves memory impairment, cholinergic dysfunction, and oxidative stress damage in scopolamine-treated rats

BMC Complementary and Alternative Medicine

Ciobica Alin³  Emmanuel Asongalem Acha⁴  Eglantine Wado²  Hervé Hervé Ngatanko Abaïssou¹  Harquin Simplice Foyet⁵ 

[1] Department of Life and Earth Sciences, Higher Teachers’ Training College, University of Maroua, Maroua, P.O. Box: 55, Cameroon;Department of Agriculture, Cattle farming and Derived products, High Institute of the Sahel, University of Maroua, Maroua, P.O. Box: 46, Cameroon;Center of Biomedical Research of the Romanian Academy, Iasi Branch, Romania;Department of Biomedical Science, Faculty of Health Sciences, University of Buea, Buea, P.O. Box 63, Cameroon;Department of Biological Sciences, Faculty of Science, University of Maroua, Cameroon. P.O. Box: 814, Maroua, Cameroon

关键词: Spatial memory;    Acetylcholine;    Antioxidant;    Scopolamine;    Emilia coccinae;   

Others  :  1232988
DOI  :  10.1186/s12906-015-0864-4

 received in 2015-05-06, accepted in 2015-09-16,  发布年份 2015

【 摘 要 】

Background

E. coccinae (SIMS) G. (Asteraceae) is an annual plant commonly found throughout the plain of the Central Africa and widely used in Cameroonian folk medicine for the treatment of fever and convulsions in children. We previously reported that the methanolic extract of this plant improved spatial memory. However no underlying mechanism was explored. The present study was undertaken to investigate the effects of the hydroalcoholic extract of Emilia coccinae on memory in scopolamine treated rats and to propose possible mechanisms of action.

Methods

Novel object recognition and Y-maze paradigm were used to test memory while oxidative profile, AChE and ACh level of the whole brain were assessed to outline the mechanism of nootropic activity of the extract. 200 and 400 mg/kg of the extract were chronically administrated during 14 consecutive days in separate groups of scopolamine intraperitoneal treated rats (1.5 mg/kg).

Results

The hydroalcoholic extract of Emilia coccinae (HEEC) at the dose of 200 mg/kg significantly improved the memory of rats and reversed the amnesia induced by scopolamine. In addition, we showed that this extract is decreasing the acetyl cholinesterase activity while also increasing the acetylcholine levels in the brain. HEEC (200 and 400 mg/kg) significantly increased antioxidant enzyme activities (SOD, GSH and CAT) and reduced lipid peroxidation (MDA level) in the rat whole brain homogenates.

Conclusions

Taken together, our results suggested that the hydroalcoholic extract of Emilia coccinae ameliorated the cognitive dysfunction in scopolamine treated rats through the blockage of the oxidative effect of scopolamine and inhibition of AChE activity.

【 授权许可】

   
2015 Foyet et al.

【 图 表 】

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