The role of the skin barrier in modulating the effects of common skin microbial species on the inflammation, differentiation and proliferation status of epidermal keratinocytes

BMC Research Notes

Georgios N Stamatas¹  Thierry Oddos²  Diaraf Yaradou²  José Serrano²  Heng Kuan Wong²  Patrick Duckney² 

[1] Johnson & Johnson Santé Beauté France, 1 rue Camille Desmoulins, Issy-les-Moulineaux 92787, France;Johnson & Johnson Santé Beauté France, Campus de Maigremont, Val de Reuil 27100, France

关键词: Inflammation;    Keratinocyte differentiation;    Skin microbiome;    Reconstructed human epidermis;    Stratum corneum;    Skin barrier;    Microbial infection;   

Others  :  1140746
DOI  :  10.1186/1756-0500-6-474

 received in 2013-04-24, accepted in 2013-11-12,  发布年份 2013

【 摘 要 】

Background

Skin resident microbial species are often thought of either as pathogenic or commensal. However, little is known about the role of the skin barrier in modulating their potential for causing disease. To investigate this question we measured the effects of three microbial species commonly found on the skin (Staphylococcus epidermidis, Staphylococcus aureus, and Propionibacterium acnes) on a reconstructed human epidermal model by either applying the bacteria on the model surface (intact barrier) or adding them to the culture medium (simulating barrier breach).

Results

When added to the medium, all of the tested species induced inflammatory responses and keratinocyte cell death with species-specific potency. P. acnes and S. epidermidis induced specific alterations in the expression of keratinocyte differentiation and proliferation markers, suggesting a barrier reparation response. S. aureus induced complete keratinocyte cell death. On the contrary, topically applied S. epidermidis and P. acnes caused no inflammatory response even when tested at high concentrations, while topical S. aureus induced a weak reaction. None of the tested species were able to alter the expression of keratinocyte differentiation or expression markers, when applied topically.

Conclusions

We show that the skin barrier prevents the effects of common skin bacteria on epidermal keratinocyte inflammation, differentiation and proliferation and highlight the importance of skin barrier in defending against the pathogenic effects of common skin bacteria.

【 授权许可】

   
2013 Duckney et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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