Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation

BMC Developmental Biology

S. Randal Voss¹  David M. Gardiner²  Charless Fowlkes³  Carlos Diaz-Castillo²  Antony Athippozhy¹  Catherine D. McCusker⁴ 

[1] Department of Biology, Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington 40506, KY, USA;Department of Developmental and Cellular Biology, University of California, Irvine 92602, CA, USA;Donald Bren School of Information and Computer Science, University of California, Irvine 92602, CA, USA;Department of Biology, University of Massachusetts, Boston 02125, MA, USA

关键词: Microarray;    Extracellular matrix;    Differentiation;    Intercalation;    Plasticity;    Positional information;    Limb regeneration;   

Others  :  1234323
DOI  :  10.1186/s12861-015-0095-4

 received in 2015-08-21, accepted in 2015-11-16,  发布年份 2015

【 摘 要 】

Background

The endogenous ability to dedifferentiate, re-pattern, and re-differentiate adult cells to repair or replace damaged or missing structures is exclusive to only a few tetrapod species. The Mexican axolotl is one example of these species, having the capacity to regenerate multiple adult structures including their limbs by generating a group of progenitor cells, known as the blastema, which acquire pattern and differentiate into the missing tissues. The formation of a limb regenerate is dependent on cells in the connective tissues that retain memory of their original position in the limb, and use this information to generate the pattern of the missing structure. Observations from recent and historic studies suggest that blastema cells vary in their potential to pattern distal structures during the regeneration process; some cells are plastic and can be reprogrammed to obtain new positional information while others are stable. Our previous studies showed that positional information has temporal and spatial components of variation; early bud (EB) and apical late bud (LB) blastema cells are plastic while basal-LB cells are stable. To identify the potential cellular and molecular basis of this variation, we compared these three cell populations using histological and transcriptional approaches.

Results

Histologically, the basal-LB sample showed greater tissue organization than the EB and apical-LB samples. We also observed that cell proliferation was more abundant in EB and apical-LB tissue when compared to basal-LB and mature stump tissue. Lastly, we found that genes associated with cellular differentiation were expressed more highly in the basal-LB samples.

Conclusions

Our results characterize histological and transcriptional differences between EB and apical-LB tissue compared to basal-LB tissue. Combined with our results from a previous study, we hypothesize that the stability of positional information is associated with tissue organization, cell proliferation, and pathways of cellular differentiation.

【 授权许可】

   
2015 McCusker et al.

【 图 表 】

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