Evaluation of the cytotoxic effect and antibacterial, antifungal, and antiviral activities of Hypericum triquetrifolium Turra essential oils from Tunisia

BMC Complementary and Alternative Medicine

Mahjoub Aouni⁴  Guido Flamini²  Pier Luigi Cioni²  Ameur Elaissi¹  Thabet Yangui⁵  Sadok Koudja³  Nabil Abid⁴  Zyed Rouis⁴ 

[1] Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia;Dipartimento di Chimica Bioorganica e Biofarmacia, Universita’ di Pisa, Via Bonanno 33, Pisa 56126, Italy;Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, Monastir 5000, Tunisia;Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia;Laboratoire des Bioprocédés, Pôle d’Excellence Régionale AUF, (PER-LBP) Centre de Biotechnologie de Sfax, BP: 1177, Sfax 3018, Tunisia

关键词: Fungi;    Bacteria;    Essential oils;    Coxsakievirus B3;    Hypericum triquetrifolium;   

Others  :  1231151
DOI  :  10.1186/1472-6882-13-24

 received in 2012-04-04, accepted in 2013-01-23,  发布年份 2013

【 摘 要 】

Background

A number of bio-active secondary metabolites have been identified and reported for several Hypericum species. Many studies have reported the potential use of the plant extracts against several pathogens. However, Hypericum triquetrifolium is one of the least studied species for its antimicrobial activity. The aim of the present study was to evaluate the cytotoxic effect of the essential oils of Hypericum triquetrifolium as well as their antimicrobial potential against coxsakievirus B3 and a range of bacterial and fungal strains.

Methods

The essential oils of Hypericum triquetrifolium harvested from five different Tunisian localities (Fondouk DJedid, Bou Arada, Bahra, Fernana and Dhrea Ben Jouder) were evaluated for their antimicrobial activities by micro-broth dilution methods against bacterial and fungal strains. In addition, the cytotoxic effect and the antiviral activity of these oils were carried out using Vero cell lines and coxsakievirus B3.

Results

The results showed a good antibacterial activities against a wide range of bacterial strains, MIC values ranging between 0.39-12.50 mg/ml and MBC values between 1.56-25.0 mg/ml. In addition, the essential oils showed promising antifungal activity with MIC values ranging between 0.39 μg/mL and 12.50 μg/mL; MFC values ranged between 3.12 μg/mL and 25.00 μg/mL; a significant anticandidal activity was noted (MIC values comprised between 0.39 μg/mL and 12.50 μg/mL). Although their low cytotoxic effect (CC₅₀ ranged between 0.58 mg/mL and 12.00 mg/mL), the essential oils did not show antiviral activity against coxsakievirus B3.

Conclusion

The essential oils obtained from Hypericum triquetrifolium can be used as antimicrobial agents and could be safe at non cytotoxic doses. As shown for the tested essential oils, comparative analysis need to be undertaken to better characterize also the antimicrobial activities of Hypericum triquetrifolium extracts with different solvents as well as their purified fractions and their pure secondary metabolites.

【 授权许可】

   
2013 Rouis et al.; licensee BioMed Central Ltd.

【 图 表 】

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