Highly efficient method for isolation of total RNA from adipose tissue

BMC Research Notes

Susanna Cirera¹ 

[1] Department of Veterinary Clinical and Animal Sciences, Section for Animal Genetics, Bioinformatics and Breeding, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark

关键词: RNA integrity;    RNA purity;    Total RNA isolation;    Adipose tissue;   

Others  :  1140748
DOI  :  10.1186/1756-0500-6-472

 received in 2013-01-03, accepted in 2013-11-01,  发布年份 2013

【 摘 要 】

Background

RNA extraction is a crucial step for monitoring gene expression. Poor RNA quality (including degradation and remaining impurities) can result in misleading results. Isolation of RNA from animal tissues with high lipid content can be challenging. Especially, it is not trivial to isolate high quality RNA with a reasonable yield from adipose tissue. The aim of this study was to provide an optimized protocol for isolating total RNA from adipose tissue. This was achieved by combining the advantages of the two routinely used methods, TRI Reagent® and miRNeasy.

Findings

The miRNeasy method results in cleaner samples but more prone to degradation while the TRI Reagent® method results in samples contaminated with salts and solvents but more intact. The new protocol combines the best of both methods resulting in RNA of high quality and suitable for downstream experiments like RT-qPCR, microarrays and high-throughput sequencing.

Conclusions

The current protocol for total RNA isolation from adipose tissue yields sufficient amount of high quality total RNA free of contaminants.

【 授权许可】

   
2013 Cirera; licensee BioMed Central Ltd.

【 图 表 】

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