Gastroprotective effect of carob (Ceratonia siliqua L.) against ethanol-induced oxidative stress in rat

BMC Complementary and Alternative Medicine

Lamjed Marzouki³  Mohamed Amri¹  Jamel El-Benna⁴  Hichem Sebai²  Abdelaziz Souli¹  Slimen Selmi³  Mohamed Amine Jabri²  Kaïs Rtibi³ 

[1] Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis. Campus Universitaire El Manar II, Tunis 2092, Tunisia;Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia;Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Resssources, Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P, 382-9000, Béja, Tunisia;INSERM, U1149, Centre de Recherche Sur l’Inflammation, Faculté de Médecine X. Bichat, Paris, 75018, France

关键词: Rat;    Antioxidant capacity;    Oxidative stress;    Carob pods;    Gastric ulcer;   

Others  :  1222524
DOI  :  10.1186/s12906-015-0819-9

 received in 2015-02-07, accepted in 2015-08-11,  发布年份 2015

【 摘 要 】

Background

We aimed in the present study, at investigating the gastroprotective effect of carob pods aqueous extract (CPAE) against ethanol-induced oxidative stress in rats as well as the mechanism implicated.

Methods

Adult male wistar rats were used and divided into six groups of ten each: control, EtOH (80 % v/v, 4 g/kg b.w.), EtOH 80 % + various doses of CPAE (500, 1000 and 2000 mg/kg, b.w.) and EtOH + Famotidine (10 mg/kg, p.o.) Animals were perorally (p.o.) pre-treated with CPAE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) for two hours.

Results

The colorimetric analysis demonstrated that the CPAE exhibited an importance in vitro antioxidant activity against ABTS and DPPH radicals. We found that CPAE pretreatment in vivo, protected against EtOH-induced macroscopic and histological changes induced in stomach mucosa. Carob extract administration also protected against alcohol-induced volume gastric juice decrease. More importantly, We showed that CPAE counteracted EtOH-induced gastric lipoperoxidation, reversed the decrease of sulfhydryl groups (−SH) an hydrogen peroxide (H ₂ O ₂ ) levels, and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).

Conclusions

These findings suggest that CPAE exerted a potential gastro-protective effect against EtOH-induced oxidative stress in rats, due in part, to its antioxidants properties.

【 授权许可】

   
2015 Rtibi et al.

【 图 表 】

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