Invasion of Solanum tuberosum L. by Aspergillus terreus: a microscopic and proteomics insight on pathogenicity

BMC Research Notes

Narayan Chandra Talukdar²  Sharma K Chandradev²  Mohendro Wakambam Singh²  Pardeep Kumar Bhardwaj¹  Pranab Roy³  Sayanika Devi Waikhom²  Bengyella Louis⁴ 

[1] Regional Centre of Institute of Bioresources and Sustainable Development (RCIBSD), Gangtok 737102, Sikkim, India;Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal 795001, Manipur, India;Department of Biotechnology, Haldia Institute of Technology, Haldia 721657, West Bengal, India;Department of Biochemistry, University of Yaoundé I, BP812-Yaoundé, Yaoundé, Cameroon

关键词: Scanning electron microscopy;    Class I patatin;    Lipoxygenase;    Stomata atropism;    Multipolar conidia germination;    Proteome;    Opposing accessory conidia;   

Others  :  1132529
DOI  :  10.1186/1756-0500-7-350

 received in 2014-02-10, accepted in 2014-06-04,  发布年份 2014

【 摘 要 】

Background

Aspergillus terreus is one of the most harmful filamentous fungal pathogen of humans, animals and plants. Recently, researchers have discovered that A. terreus can cause foliar blight disease in potato (Solanum tuberosum L.). We used light and scanning electron microscopy, and performed proteomics analysis in an attempt to dissect the invasion process of A. terreus in this important crop.

Results

Microscopic study revealed that invasion of leaf tissue is marked by rapid germination of A. terreus phialidic conidia (PC) by 4 h after inoculation. By 8 h after inoculation, primary germ tubes from PC differentiated into irregular protuberance, often displayed stomata atropism, and failed to penetrate via the epidermal cells. Colonization of leaf tissues was associated with high rate of production of accessory conidia (AC). These analyses showed the occurrence of a unique opposing pattern of AC, tissue-specific and produced on melanized colonizing hyphae during the infection of leaf tissue. A significant proteome change hallmarked by differential expression of class I patatin, lipoxygenase, catalase-peroxidase complex, and cysteine proteinase inhibitor were observed during tuber colonization. These proteins are often involved in signal transduction pathways and crosstalk in pathogenic responses.

Conclusion

A. terreus abundantly produced AC and multipolar germinating PC to invade potato leaf tissue. Additionally, A. terreus differentially induced enzymes in potato tuber during colonization which facilitates rapid disease development.

【 授权许可】

   
2014 Louis et al.; licensee BioMed Central Ltd.

【 图 表 】

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