Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions

BMC Microbiology

Shiyan Qiao¹  Hong Liu¹  Chengli Hou¹  Xiangfang Zeng¹  Aina Wang²  Fengjuan Yang¹ 

[1] State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China;Weifang Business Vocational College, Zhucheng 262234, China

关键词: Lipopolysaccharides;    IPEC-J2;    Tight junction;    Newborn piglets;    Lactobacillus;   

Others  :  1137430
DOI  :  10.1186/s12866-015-0372-1

 received in 2014-08-14, accepted in 2015-02-04,  发布年份 2015

【 摘 要 】

Background

Tight junctions (TJs) maintain the intestinal mucosal barrier, dysfunction of which plays a vital role in the pathophysiology of a variety of gastrointestinal disorders. Previously, we have shown that L. reuteri I5007 maintained the gut epithelial barrier in newborn piglets. Here we aimed to decipher the influence of L. reuteri I5007 on tight junction (TJ) protein expression both in vivo and in vitro.

Results

We found that L. reuteri I5007 significantly increased the protein abundance of intestinal epithelial claudin-1, occludin and zonula occluden-1 (ZO-1) in newborn piglets (orally administrated with 6 × 10⁹ CFU of L. reuteri I5007 daily for 14 days). In vitro, treatment with L. reuteri I5007 alone maintained the transepithelial electrical resistance (TEER) of IPEC-J2 cells with time. In addition, IPEC-J2 cells were stimulated with 1 μg/mL lipopolysaccharide (LPS) for 1, 4, 8, 12 or 24 h, following pre-treatment with L. reuteri I5007 or its culture supernatant for 2 h. The results showed that LPS time-dependently induced (significantly after 4 or 8 h) the expression of TNF-α and IL-6, and decreased TJ proteins, which was reversed by pre-treatment of L. reuteri I5007 or its culture supernatant.

Conclusions

L. reuteri I5007 had beneficial effects on the expression of TJ proteins in newborn piglets and the in-vitro results showed this strain had a positive effect on TEER of cells and inhibited the reduction of TJ proteins expression induced by LPS. These findings indicated L. reuteri I5007 may have potential roles in protection TJ proteins in TJ-deficient conditions.

【 授权许可】

   
2015 Yang et al.; licensee BioMed Central.

【 图 表 】

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