New microsatellite loci for the green sea urchin Strongylocentrotus droebachiensis using universal M13 labelled markers

BMC Research Notes

Kjell Magnus Norderhaug¹  Johannes Haugstetter²  Camilla With Fagerli³  Hege Gundersen³  Hartvig Christie³  Anders Hobæk⁴  Marc B Anglès d’Auriac³ 

[1] University of Oslo, Department of Biosciences, Box 1066, Blindern, 0316 OSLO, Norway;ecogenics GmbH, 8952 Schlieren, Switzerland;Norwegian Institute for Water Research, N-0349 Oslo, Norway;Norwegian Institute for Water Research, N-5006 Bergen, Norway

关键词: Strongylocentrotus droebachiensis;    Simple DNA preparation;    NE Atlantic;    Microsatellites;    Marine invertebrates;    Kelp forests;    Heterozygote deficiency;    Climate change;   

Others  :  1127336
DOI  :  10.1186/1756-0500-7-699

 received in 2014-03-21, accepted in 2014-10-02,  发布年份 2014

【 摘 要 】

Background

The green sea urchin Strongylocentrotus droebachiensis has a wide circumpolar distribution and plays a key role in coastal ecosystems worldwide by destructively grazing macroalgae beds and turn them into marine deserts, so-called barren grounds. In the past decades, large established kelp forests have been overgrazed and transformed to such barren grounds on the Norwegian coast. This has important repercussions for the coastal diversity and production, including reproduction of several fish species relying on the kelp forests as nurseries. Genetic diversity is an important parameter for the study and further anticipation of this large scale phenomenon.

Findings

Microsatellites were developed using a Norwegian S. droebachiensis individual primarily for the study of Northeast Atlantic populations. The 10 new microsatellite loci were amplified using M13 forward tails, enabling the use of M13 fluorescent tagged primers for multiplex reading. Among these loci, 2 acted polysomic and should therefore not be considered useful for population genetic analysis. We screened 96 individuals sampled from 4 different sites along the Norwegian coast which have shown unexpected diversity.

Conclusions

The new microsatellite loci should be a useful resource for further research into connectivity among S. droebachiensis populations, and assessing the risks for spreading and new overgrazing events.

【 授权许可】

   
2014 Anglès d’Auriac et al.; licensee BioMed Central Ltd.

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