Functional annotation and ENU

BMC Research Notes

Teresa M Gunn¹ 

[1]McLaughlin Research Institute, 1520 23rd St S, Great Falls, MT, 59405, USA

关键词: Functional annotation;    Mouse genetics;    Mutagenesis;    ENU;    Ethylnitrosourea;   

Others  :  1165422
DOI  :  10.1186/1756-0500-5-580

 received in 2012-10-09, accepted in 2012-10-09,  发布年份 2012

【 摘 要 】

Functional annotation of every gene in the mouse genome is a herculean task that requires a multifaceted approach. Many large-scale initiatives are contributing to this undertaking. The International Knockout Mouse Consortium (IKMC) plans to mutate every protein-coding gene, using a combination of gene trapping and gene targeting in embryonic stem cells. Many other groups are performing using the chemical mutagen ethylnitrosourea (ENU) or transpon-based systems to induce mutations, screening offspring for phenovariants and identifying the causative mutations. A recent paper in BMC Research Notes by Arnold et al. presents data from an ENU-based mutagenesis project that provides not only some of the first phenotype-genotype information for a large number of genes, but also a trove of information, all publicly available, that demonstrates the specificity and efficiency of ENU mutagenesis.

【 授权许可】

   
2012 Gunn; licensee BioMed Central Ltd.

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