Drug resistance-conferring mutations in Mycobacterium tuberculosis from Madang, Papua New Guinea

BMC Microbiology

Hans-Peter Beck⁴  Sebastien Gagneux⁴  Suparat Phuanukoonnon¹  Peter Siba¹  Sonia Borrell⁴  Christopher Coulter²  Robyn Carter²  Stefan Niemann³  Mireia Coscolla⁴  Serej Ley¹  Paul Harino¹  Marie Ballif⁴ 

[1] Papua New Guinea Institute of Medical Research, Goroka and Madang, Papua New Guinea;Queensland Mycobacterium Reference Laboratory, Pathology Queensland, Brisbane, Australia;Borstel Research Center, Borstel, Germany;University of Basel, Basel, Switzerland

关键词: Mutations;    Drug resistance;    Papua New Guinea;    Mycobacterium tuberculosis;   

Others  :  1221773
DOI  :  10.1186/1471-2180-12-191

 received in 2012-05-14, accepted in 2012-08-06,  发布年份 2012

【 摘 要 】

Background

Monitoring drug resistance in Mycobacterium tuberculosis is essential to curb the spread of tuberculosis (TB). Unfortunately, drug susceptibility testing is currently not available in Papua New Guinea (PNG) and that impairs TB control in this country. We report for the first time M. tuberculosis mutations associated with resistance to first and second-line anti-TB drugs in Madang, PNG. A molecular cluster analysis was performed to identify M. tuberculosis transmission in that region.

Results

Phenotypic drug susceptibility tests showed 15.7% resistance to at least one drug and 5.2% multidrug resistant (MDR) TB. Rifampicin resistant strains had the rpoB mutations D516F, D516Y or S531L; Isoniazid resistant strains had the mutations katG S315T or inhA promoter C15T; Streptomycin resistant strains had the mutations rpsL K43R, K88Q, K88R), rrs A514C or gidB V77G. The molecular cluster analysis indicated evidence for transmission of resistant strain.

Conclusions

We observed a substantial rate of MDR-TB in the Madang area of PNG associated with mutations in specific genes. A close monitoring of drug resistance is therefore urgently required, particularly in the presence of drug-resistant M. tuberculosis transmission. In the absence of phenotypic drug susceptibility testing in PNG, molecular assays for drug resistance monitoring would be of advantage.

【 授权许可】

   
2012 Ballif et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1 .

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