The use of a new miniature cryoprobe for ablation of bone tissue: In vivo assessment of the probe and application of the method to bone in a sheep model

BMC Surgery

Peer Eysel¹  Dietmar-Pierree König¹  Marfalda Bosse³  Heike Erberich²  Marc Land¹  Frank Popken¹ 

[1]Department of Orthopedic Surgery, University of Cologne, Josef-Stelzmann-Str. 9, 50931 Cologne, Germany

[2]Institute of Pathology, University of Cologne, Josef-Stelzmann-Str. 9, 50931 Cologne, Germany

[3]Institute of Experimental Medicine, University of Cologne, Robert-Koch-Str. 10, 50931 Cologne, Germany

关键词: bone tumour;    bone;    miniature cryoprobe;    cryosurgery;   

Others  :  1123817
DOI  :  10.1186/1471-2482-3-3

 received in 2002-11-17, accepted in 2003-04-22,  发布年份 2003

【 摘 要 】

Background

So far, modern miniature cryoprobes were used for local destruction of soft tissue tumours without damaging the adjacent healthy tissue. In this study, cryoablation methodology was applied to bone and the cooling capacity of the probe was examined in vitro and in vivo.

Method

Freezing was performed by cooling one or two probes (diameter 3.2 mm) to -180°C with liquid nitrogen. The cooling capacity of the probes was determined optically and thermally against a homogeneous reference gel, followed by in vivo measurements on femoral and tibial sheep bone followed by histological examination.

Results

Thanks to the synergistic effect, the simultaneous use of 2 probes produced an almost spherical expansion of cold in the homogenous gelatin. During the in vivo freezes, the temperature curves showed a more moderate trend. Nevertheless, due to the synergistic effect, temperatures below -50°C could be reached at a distance of 1 cm from the probe. No local or systemic intraoperative complications were observed. Histological examination revealed cell necrosis up into the -10°C isotherm.

Conclusions

Adequate tissue cooling of the bone matrix can be achieved with in vivo freezes by means of one or more miniature cryoprobes. Therefore, this probe could provide an alternative to or supplement surgical resection of pathological bone processes.

【 授权许可】

   
2003 Popken et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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